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Will a folded resolution IV design result in a higher resolution design?
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- Calculate the scan time for a GRE with TR = 30 msec, NEX = 2, Ny = 256, for (a) a single slice and (b) 15 slices.You may want to use this resource for this problem. If you do, submit the output along with your solution.You have been given a confocal microscope equipped with the following lasers, excitation filters, andemission filters:Laser Emission filter355 nm 410-470 nm405 nm 470-500 nm488 nm 500-550 nm532 nm 570-610 nm561 nm 610-650 nm640 nm 660-700 nm808 nm 720-780 nmYour task is to design an experiment to visualize the following:1. Nuclei2. A fluorescent protein in the cytosol3. A cell membrane marker antibody conjugated with a fluorophore4. Actin filaments5. LysosomesYou may choose from the following fluorophores for each of the five channels:Nuclei Fluorescent protein Membrane marker Actin marker Lysosome trackerDAPI GFP FITC AF488 Phalloidin LysoTracker RedHoechst 33342 YFP WGA-TRITC AF568 Phalloidin LysoTracker DeepRedSYTO Deep Red RFP Cy7 AF594 Phalloidin LysoTracker Blue Part 3.1Choose appropriate fluorophores for each of the subcellular structures to be imaged, taking into…How many coccus cells (0.0005 mm in diameter) could fit across the entire FOV of the 100x objective lens (assuming the DFOV of the 100x lens is 180 μm?
- In your opinion, for efficient multiphoton excited fluorescence microscopy, would it be better to use a 100 fs 80 MHz laser pulse train or a 200 fs 80 MHz laser pulse train for imaging biological samples? Please explain your answer using equations 1 and 2 <I(t)2> = gP <I(t)>2 / (Rτ) ..............equa (1) τout = τin (1 + 7.68(D/τ2in)2) 1⁄2 ................equa (2) D is the total dispersion in femtoseconds squared gp is a unitless factor that depends on the temporal laser pulse shape (0.66 for a Gaussian pulse shape), τ is the full-width half-maximum (FWHM) of time average valueWhat is the diameter of the field of view (DFV) of a 1000x objective lens if the DFV of a 400x objective lens is 500 μ? Express your answer in mm.What is the difference between low reolution and high resolution?
- Why is the wavelength of the light that is released in spectrophotometric analysis longer than the wavelength of the light that is used to activate a fluorescent molecule?What is FRAP (Fluorescence Recovery After Photobleaching)? Draw a graph of fluorescence versus time during FRAP. What does FRAP prove?Discuss the principles and applications of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry in molecular diagnostics.