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Which is the best method for producing phase-separated GUVs (at least 20 um in size)?
Giant unilamellar vesicles (GUVs) are used as model membrane systems whose sizes are very similar to cells
GUVs are used to study the function of highly complex bio-membranes with heterogeneous lipid composition, structure, mechanical and chemical properties.
To prepare GUVs with a complex lipid mixture one needs to mix in two heterogeneous populations of small unilamellar vesicles
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- With an A260 value of 1.12 and a DNA concentration of 56.0 ng/mL, what is the pathlength of the optical system used within the Nanodrop?a) What are the important parameters for the eluent selection in Gel Permeation Chromotography (GPC)? Explain your answer in detail. What is an universal calibration curve and what is the advantage of using it in GPC analysis?A mixture of species of A and B is injected into a liquid chromatographic column with length of 20.0 cm. The obtained separation results are listed as follows: Species Retention time (min) Peak Width at Base (min) Non-retained 3.0 A 5.2 0.48 В 5.9 0.56 From the data, the calculated resolution Rs is smaller than 1.5 for A and B when the column length is 20.0 cm, which indicates that A and B are not separate completely. What is the new column length (unit: cm) necessary to separate the A and B species with a good resolution Rs = 1.5? Please keep your answer to one decimal place.
- For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. What are the initial concentrations used for tubes 3, 5, and 6? Show pertinent solutions. What are the dilutions of the last positive tube and first negative tube respectively? Show computation.You perform a Bradford assay to determine the concentration of isolated α-lactalbumin. You use 50 μL of a two-fold diluted solution of α-lactalbumin in the assay. You generate a standard curve with the following equation for the line: y = 0.163x + 0.082. The absorbance of your sample was 0.674 AU. What is the concentration of α-lactalbumin, in mg/mL, in your sample? Give your answer to three significant figures.The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?
- What is The Luria-Delbrück fluctuation experiment?For the same chromatographic column and experimental conditions Rs when H = 3 cm = Rs when H = 5 cm Rs when H = 3 cm > Rs when H = 5 cm Rs when H = 3 cm < Rs when H = 5 cm The two quantities cannot be compared due to insufficient information.How many microliters of the 500mM colchicine is required for making 1.5mL cell culture with 2mM colchione?