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Basic Clinical Laboratory Techniques 6E
Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter3: Basic Hemostasis
Section3.2: Disorders Of Hemostasis
Problem 11RQ
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Procedure Take ml Top up to 50ml. Add 50 μι of Mix Sml mixture to Add fresh LB ampicillin to the overnight culture with 45 ml LB mixture analyse OD600 the both broth Measure every 15 mins. Stop until 75 mins. The mixture is then incubated Withdraw 3ml mixture to analyse (OD600)record as zero time-point. Overnight culture (E-coli + LB borth) at shaked incubator at 37°c 200rpm Mix overnight culture B (control) with 45 ml LB Sml Take 3 ml No ampicillin No ampicilin mixture analyse OD600 to the broth The mixture is then Withdraw 3ml mixture to Measure every 15 mins. Stop until incubated incubator at 37°c 200rpm shaked analvse (OD600)record as zero time-point. at 75 mins. Graph of Absorbance vs Time(mins) 14 12 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) Group 1(Ampicilin) Group 1(Control) Measurement of bacterial cell growth in LB Absorbance(600nm)
Transcribed Image Text:Procedure Take ml Top up to 50ml. Add 50 μι of Mix Sml mixture to Add fresh LB ampicillin to the overnight culture with 45 ml LB mixture analyse OD600 the both broth Measure every 15 mins. Stop until 75 mins. The mixture is then incubated Withdraw 3ml mixture to analyse (OD600)record as zero time-point. Overnight culture (E-coli + LB borth) at shaked incubator at 37°c 200rpm Mix overnight culture B (control) with 45 ml LB Sml Take 3 ml No ampicillin No ampicilin mixture analyse OD600 to the broth The mixture is then Withdraw 3ml mixture to Measure every 15 mins. Stop until incubated incubator at 37°c 200rpm shaked analvse (OD600)record as zero time-point. at 75 mins. Graph of Absorbance vs Time(mins) 14 12 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) Group 1(Ampicilin) Group 1(Control) Measurement of bacterial cell growth in LB Absorbance(600nm)
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