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procedure/s in performing aseptic transfer of bacterial cultures in (include illustration)
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- procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (3) agar plate to agar slant.procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (1) broth culture to brothWhy should agar media be completely dissolved before they are dispensed in tubes and plates? What are the bases for pegging the temperature at 1210C for 15-30 minutes during moist heat sterilization and 1800C for two (2) hours using dry heat sterilization? Can you sterilize culture media using dry heat sterilization? Why is that so? You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in media prep, sterilization and culture of microorganisms? Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it technically acceptable in microbiology?
- during inoculation, the bacterial culture tube is always held at an angle and the lid of the Petri dish is slightly open. Explain the purpose of these steps briefly.Drawings of the two bacterial cultures as observed under the 4 mm objective. Describe features observable under the high-power objective and their movement as seen in the hanging drop preparationIn aseptic technique, what will happen if the inoculated solid plated media is not inverted prior to incubation?
- If you have a 15 mg/100 ml stock solution of GA3 and you need a 1 mg GA3 in 25 ml, how much stock solution would you add to 125 ml of medium? how to calculate these kind of question in tissue culture media preperationYou are tasked to observe the motility of an UNKNOWN Bacteria isolated from a mixed culture, which technique/s is BEST to perform? How shall the procedure be carried out? Explain.Blood agar is often used to observe changes in the appear-ance of the agar around the colonies growing on this medium.This medium could then be called:(a) Selective(b) Designated(c) Differential(d) Defined(e) Exact
- Isolating a pure culture from a mix sample is typically done on solid agar but can be performed using a liquid media. How can you isolate a pure culture from a mixed culture in a liquid medium (broth, not agar)?In a fed-batch culture operating with intermittent addition of lactose solution, values ofthe following parameters are given at time t = 2, when the system is at a quasi-steady state. 1.Determine the initial volume of the culture 2. Determine the concentration of growth-limiting substrate and the total amount ofbiomass in the vessel at a quasi-steady state. 3. At which scenario of bioprocessing, a fed-batch system is recommended to be applied?In performing the Kirby-Bauer procedure in a clinical laboratory setting, why must the agar be a certain depth? Why must the absorbance of the inoculum be standardized?