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Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book.
Explore the Bicarbonate/Carbonic Add Buffering System of Blood Plasma At 37°C, if the plasma pH is 7.4 and the plasma concentration of HCO3]- is 15 m M, what is the plasma concentration of H2CO3? What is the plasma concentration of
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Biochemistry
- Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Quantitative Relationships Between Rate Constants to Calculate Km, Kinetic Efficiency (kcat/Km) and Vmax - IV Carbonic anhydrase catalyzes the hydration of CO2: CO2+H2OH2CO3The Km of carbonic anhydrase for CO2is 12 mM. Carbonic anhydrase gave an initial velocity v0=4.5molH2CO3formed/mLsecwhen [CO2] = 36 mM. a. What is Vmax for this enzyme? b. Assuming 5 pmol/mL (510-12moles/mL) of enzyme were used in this experiment, what is kcat for this enzyme? c. What is the catalytic efficiency of this enzyme? d. Does carbonic anhydrase approach catalytic perfection?arrow_forwardAnswers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. How to Prepare a Buffer Solution: An Anserine Buffer Draw the titration curve for anserine (Figure 2.16). The isoelectric point of anserine is the pH where the net charge on the molecule is zero: what is the isoelectric point for anserine? Given a 0.1 M solution of anserine at its isoelectric point and ready access to 0.1 M HCl, 0.1 M NaOH, and distilled water, describe the preparation of 1 L of 0.04 M anserine buffer solution. pH 7.2.arrow_forwardAnswers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Preparing a Phosphate Buffer Solution of pH 7.5 from Solutions of Na3PO4 and H3PO4 Given 0.1 M solutions of Na3PO4, and H3PO4 describe the preparation of 1 L of a phosphate buffer at a pH of 7.5. What are the molar concentrations of the ions in the final buffer solution, including Na+ and H+?arrow_forward
- Answers to all problems are at the end οΓthis book. Detailed solutions are available in the Student Solutions Manual. Study Guide, and Problems Book. Consult the following reference (Samanta U. and Bahnson. B. J., 2008. Crystal structure of human plasma platelet-activating factor acetylhydrolase. Journal of Biological Chemistry 283:31617-3U624). consider the active-site structure of this enzyme in the Light of material in this chapter, and write a detailed mechanism for the PAF acetylhydrolase.arrow_forwardAnswers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Calculate the pH Change in a Phosphate Buffer When Acid or Base Is Added If 50 mL of 0.01 M HCL is added to 100 mL of 0.05 M phosphate buffer at pH 7.2, what is the resultant pH? What are the concentrations of H2POh- and HPO42- in the final solution? If 50 mL of 0.01 M NaOH is added to 100 mL of 0.05 M phosphate buffer at pH 7.2, what is the resultant pH0 What are the concentrations of H2P04- and HPO42- in this final solution?arrow_forwardAnswers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Calculating the pH of a Solution of a Weak Acid; Calculating the pH of the Solution after the Addition of Strong Base The ka for formic acid is 1.78 10-4 M. What is the pH of a 0.1 M solution of formic acid? 150 mL of 0.1 M NaOH is added to 200 mL of 0.1 M formic acid, and water is added to give a final volume of 1 L. What is the pH of the final solution?arrow_forward
- Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Plot the Titration Curve for Bicine and Calculate How to Prepare a pH 7.5 Bicine Buffer Solution Bicine (N, N—bis (2-hydroxyethyl) glycine) is another commonly used buffer in biochemistry labs. The structure of Bicine in its fully protonated form is shown here: Draw the titration curve for Bicine. assuming the pA'a for its free COOH group is 2.3 and the pAa for its tertiary amino group is 8.3. Draw the structure of the fully deprotonated form (completely dissociated form) of bicine. You have available a U.l Msolution of Bicine at its isoelectric point (pH|)T 0.1 M solutions of HCI and NaOH. and ample distilled water. Describe the preparation of 1 L of 0.U4 M Bicine buffer. pH 7.5. What is the concentration of the fully protonated form of Bicine in your final buffer solution?arrow_forwardAnswers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Exploring the Michaelis-Menten Equation - I According to the Michaelis-Menten equation, what is the v/Vmax ratio when [S] = 4 Km?arrow_forwardAnswers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Polyprotic Acids: Citric Acid Species at Various pHs Citric acid, a tricarboxylic acid important in intermediary metabolism, can be symbolized as H3A. Its dissociation reactions are H3AH++H2ApK1=3.13H2AH++HA2pK2=4.76HA2H++A3pK3=6.40 If the total concentration of the acid and its anion forms is 0.02 M. what are the individual concentrations of H3A, H2A-, HA2-, and A3- at pH 5-2?arrow_forward
- Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Prepare a Buffer by Combining a Solution of Weak Acid with a Solution of the Salt of the Weak Acid Given 0.1 M solutions of acetic acid and sodium acetate, describe the preparation of 1 L of 0.1 M acetate buffer at a pH of 5.4.arrow_forwardAnswers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. How to Prepare a Buffer Solution: a HEPES Buffer Given a solution of0.1 M HEPES in its fully protonated form and ready access to 0.l M HCl, 0.1 M NaOH, and distilled water, describe the preparation of 1 L of 0.025 M HEPES buffer solution, pH 7.8.arrow_forwardAnswers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book. Determining the Electrochemical Potential of a Sodium Ion Gradient Consider a phospholipid vesicle containing 10mM Na+ ions. The vesicle is bathed in a solution that contains 52mM Na+ ions, and the electrical potential difference across the vesicle membrane = outside- outside = -30mV. What is the electrochemical potential at 25C for Na+ ions?arrow_forward
- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning