(a)
Interpretation:
The primers frequently chosen that have high number of cytosines and guanines in their sequences; the reason has to be predicted.
Concept introduction: Cytosine is a
Primers are short strand(
(b)
Interpretation:
The primer sequence of CCCCCCCCCCGGGGGGGGGG not is a good choice; the reason has to be predicted.
Concept introduction: Cytosine is a nucleotide found in DNA and RNA. It is a derivative of pyrimidine with heterocyclic aromatic ring. Guanine is the one of the four bases in DNA and RNA. It is dervative of purine. Guanine always pairs with cytosine. Guanine and Cytosine makes three hydrogen bonds.
Primers are short strand(
(c)
Interpretation:
Vast excess of primer used for the amount of DNA has to be amplified.
Concept introduction: Cytosine is a nucleotide found in DNA and RNA. It is a derivative of pyrimidine with heterocyclic aromatic ring. Guanine is the one of the four bases in DNA and RNA. It is dervative of purine. Guanine always pairs with cytosine. Guanine and Cytosine makes three hydrogen bonds.
Primers are short strand(
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Chemistry & Chemical Reactivity
- Important steps in sequencing pure proteins include all of these except: a. Determining the amino acids on the ends of the smaller peptides b. Determining the amino acids on the ends of the protein c. Determining the isoionic pH of the protein d. Determining the amino acid composition e. Breaking the protein into smaller peptides It is impossible to sequence a protein if you do not have overlapping sequences to work with. A. True B. Falsearrow_forwardThe basic principle that underlies northern and Southern blots is . A. laser excitation B. hybridization via base pairing C. fluorescently labeled antibodies D. dideoxynucleotide chain terminationarrow_forward1. Draw the two Watson-Crick Bases Pairs and show how G- C and A-T recognize each other by hydrogen bonding. 2. Draw the full structure of two nucleotides - one from DNA and one from RNA. Point out the key difference between the two. 3. Write out an example of a condensation reaction forming a phosphate-sugar bond. 4. Write out an example of a condensation reaction between a nucleic acid base and a sugar. 5. State the "Central Dogma" of biochemistry.arrow_forward
- Q23: Dr. Alghe has just performed a restriction digest on DNA she extracted from her samples. She must now run her DNA out on a gel to separate the fragments based on size. To do this she must prepare a 1.1% agarose gel, where the percentage is determined as mass/vol. How many grams of agarose must she add to 413 mL of buffer in order to arrive at the correct percentage? (answer to 2 decimals) **Assume the dissolution of agarose will not alter the final volume of the solution**arrow_forward1. How does real-time polymerase chain reaction work?2. How is SYBR Green used in the experiment?arrow_forward7. Consider the DNA segment 5'-ATGACGGTTTACTAAGCC-3' a. Write the mRNA sequence that would be obtained from the segment's transcription.arrow_forward
- 3. Tm is the temperature at which all of a certain DNA issingle-stranded. a. true b.false 4. Type II restriction endonuclease is useful for molecular cloning. a. true b.falsearrow_forwardWhat are annulenes ?arrow_forward16. Describe the basic principles behind the functioning of an amino acid analyzer. What is it? W- is it used for? How does it work (start to finish)? 17. In peptide, protein chemistry, what does the term 'sequence' mean? 18. About how many amino acid residues can be in a peptide in order to sequence it by Edman degradation?arrow_forward
- Which of the following amino acid has an aromatic group? * (2 Points) valine O lysine threonine O tyrosinearrow_forwardProtein sequence is always read (blank) terminal to (blank) terminal. And DNA sequence is always read (blank) prime to ( blank) prime. I need help filling in the blanks please? ? Thanksarrow_forwardWhat is qrxn?arrow_forward
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