Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 47, Problem 1CT
S. salivarius forms large mucoid colonies on sucrose blood agar. Would you expect the same type of colonies on glucose blood agar? Briefly explain your answer.
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Describe the appearance of an S. agalactiae colony grown on blood agar. Describe how that colony would differ in appearance from a colony of S. pyogenes.
Examine the graph shown below of a liquid culture growth curve for S. pneumoniaegrowing in minimal broth containing both glucose and lactose in equal concentrations as a potential carbon source. Answer the following questions:
a) Name the growth phases labelled as I, II and III, explain the observed pattern of growth at each of these stages in the curve
b) Where would the concentration of cAMP be low and why?
c) In what phase would β-galactosidase be high and why?
d) What kind of overall growth pattern does this curve illustrate?
Differentiate the growth curve exhibited by the same inoculum under the following conditions. Draw and label the parts of your growth curve.
A). If it is exponentially growing and inoculated in the same nutritious medium under the same optimum conditions.
B). If it is taken from an old culture and inoculated into the same nutritious medium and incubated under the same optimum conditions in (A).
C). If it is taken from an old culture and inoculated to a different and less nutritious medium and incubated under the same optimum conditions as (A).
Chapter 47 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
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