Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Textbook Question
Chapter 8, Problem 19PDQ
If a single bacteriophage infects one E. coli cell present in a culture of bacteria and, upon lysis, yields 200 viable viruses, how many phages will exist in a single plaque if three more lytic cycles occur?
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If a single bacteriophage infects one E. coli cell present on a lawn of bacteria and, upon lysis, yields 185 viable viruses, how many phages will exist in a single plaque if 5 more lytic cycles occur?
Express your answer using three significant figures (example: 1.11×10^3).
Which of the following terms describes bacteriophage DNA that has become integrated into the host cell chromosome?
A) plasmid
B) impolite phage
C) transposon
D) prophage
Why is a webbed plate capable of producing a higher lysate titer (pfu/mL) than a cleared plate?
a.) A webbed plate has plaques that have gone through more rounds of replication because the number of host cells did not limit phage replication, creating exponentially more phage particles
b.) A webbed plate has no more host available for replication, meaning the plate is full of exponentially higher amounts of phage
c.) A cleared plate has less bacteria than a webbed plate, so there are more plaques present
d.) A cleared plate contains more phage than a webbed plate because it started with a more concentrated phage sample
Chapter 8 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 8 -
CASE STUDY | To treat or not to treat
A...Ch. 8 - CASE STUDY | To treat or not to treat A...Ch. 8 - Prob. 3CSCh. 8 - Prob. 4CSCh. 8 - HOW DO WE KNOW? In this chapter, we have focused...Ch. 8 -
CONCEPT QUESTION
2. Review the Chapter Concepts...Ch. 8 -
3. Distinguish among the three modes of...Ch. 8 - With respect to F+ and F- bacterial matings, (a)...Ch. 8 - List all of the differences between F+ × F– and...Ch. 8 - Describe the basis for chromosome mapping in the...
Ch. 8 - Why are the recombinants produced from an Hfr × F–...Ch. 8 - Describe the origin of F' bacteria and...Ch. 8 -
9. Describe the mechanism of transformation.
Ch. 8 - .
10. The bacteriophage genome consists primarily...Ch. 8 - Prob. 11PDQCh. 8 - In the plaque assay, what is the precise origin of...Ch. 8 -
13. In the plaque assay, exactly what makes up a...Ch. 8 - A plaque assay is performed beginning with 1.0 mL...Ch. 8 -
15. Describe the difference between the lytic...Ch. 8 - Prob. 16PDQCh. 8 -
17. Explain the observations that led Zinder and...Ch. 8 -
18. Describe the execution of and rationale...Ch. 8 - If a single bacteriophage infects one E. coli cell...Ch. 8 - A phage-infected bacterial culture was subjected...
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- Which of the statements is TRUE about conjugation? a) Plasmid DNA is transferred from a donor (F+) cell b) It may only be performed by competent cells c) DNA is always integrated into recipient chromosome d) It may be specialized or generalizedarrow_forwardWhich of the following statements is not applicable to conjugation? A) OF factors are transferrable plasmids passed from one cell to another during conjugation. B) O During conjugation, rolling circle replication transfers an F factor from an F+ cell to an F- cell. ) O An F factor acquired during conjugation will automatically be integrated into the recipient's chromosome. D) O Conjugation requires physical contact between cells via the formation of a sex pilus. E) O An Hfr strain is capable of transferring a portion of the chromosome during conjugation.arrow_forwardWhich of the statements about bacteriophage is FALSE: a) specialized transduction is a low frequency event b) may accidently package host DNA into a phage particle c) may perform specialized transduction d) only have a lytic life cyclearrow_forward
- Which process listed below allows genetic material to be transferred from a virus-like particle that lacks genes for its own replication? A) Conjugation of an F+ plasmid B) Gene transfer through a gene transfer agent C) Transduction by a dsDNA phage Mu D) Transformation of a linear piece of DNAarrow_forwardIn conjugation of a Hfr cell with an F- cell: a) the container cell becomes F + b) The container cell remains F- c) The donor cell becomes F- d) The recipient cell becomes Hfrarrow_forward1 mL of a bacteriophage suspension is mixed with 20 mL of a bacterial culture and 50% of the phages adsorb. We know that the bacteriophage suspension had a concentration of 1x1010 viruses per mL, and the bacterial culture had a concentration of 3x108 bacteria per mL. What fraction of the cells is multiple infected?arrow_forward
- The mechanism of specialized transduction relies on the improper excision of a bacteriophage genome that is integrated in the host cell chromosom A) O True B) O Falsearrow_forwardOne mL of a bacteriophage suspension is mixed with 20 mL of a bacterialculture and 50% of the phages adsorb. We know that the bacteriophagesuspension had a concentration of 1x10^10 viruses per mL, and the bacterialculture had a concentration of 3x10^8 bacteria per mL. How many viruses are adsorbed per cell (multiplicity of infection)?arrow_forwardIn Hershey-Chase experiment, bacteriophages protein coats were tagged with radioactive isotope S-32. These phages were used to infect E. coli cells and the cells were further centrifuged to form pellets. Why was the radioactivity level of S-32 found greater outside the cells compared to the E. coli cell pellets? Explain briefly. If the experiment is repeated in the same manner but this time the phage protein coats are labelled with isotope X and the phage DNA with isotope Y, which isotope’s radioactivity will be found in greater amounts in the E. coli cell pellets after centrifugation? Explain briefly.arrow_forward
- Which of the following statements is true about a vector?a) Plasmids, phages can be used as vectorsb) All vectors are plasmidsc) Cyanobacteria can also be used as vectorsd) Fungi can also be used as vectorsarrow_forwardThere have been recent outbreaks of dog flu in the US. Why doesn't this virus infect humans? A) The virus can replicate in cells of all species but can only egress from dog cells. B) The genetic code of the virus is the same as that in dog cells but is different from other organisms. C) The virus can only attach to dog cells. D) The virus can enter cells of all species but can only replicate in dog cells.arrow_forward10 mL of a bacteriophage suspension is mixed with 5 mL of a bacterial culture and 25% of the phages adsorb. The bacteriophage suspension has a concentration of 4x1018 viruses per mL, and the bacterial culture had a concentration of 6x1014 bacteria per mL. How many viruses are adsorbed per cell (multiplicity of infection)? What fraction of the cells is uninfected? What fraction of the cells is single infected? What fraction of the cells is multiple infected?arrow_forward
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