Type-II toxin-antitoxin (TA) systems are a relatively ubiquitous feature of prokaryotic genomes (1), consisting of a ribonucleolytic toxin and a labile antitoxin. Under normal conditions, the toxin is silenced by pairing with its cognate antitoxin, but can impact cell physiology in several ways when the antitoxin is removed through proteolytic degradation. TA systems were first identified as assisting in plasmid maintenance via post-segregational killing (2,3), as well as in phage exclusion, where
GENE 222 Phylogenetic assignment Question One A. Sceptrophasma hispidulum B. Heteropteryx dilatata C. Eurycnema goliath D. Phyllium siccofolium E. Timema knulli Question Two These sequences are sourced from Subunit 1 of the Cytochrome Oxidase gene in the Mitochondria. Question Three There appears to be a greater interspecific variation along this fragment every three base pairs, which would suggest a non-random pattern. During translation of the DNA sequence, tRNA anticodons bind to DNA codons
Introduction This paper is about the analysis of a cell biology research article. In this paper, the research article we analyzed is: “The GTP-binding protein Ran/TC4 is required for protein import into the nucleus.” Ran/TC4 is described as a small nuclear GTP-binding protein that is used in the regulation of DNA synthesis (Mindong et al, 1993). Apart from aiding in the transfer of the protein into the nucleus, the Ran/TC4 has several other roles as well. Matsumoto and Beach (1991) in their study
IRE1α IRE1 exists in two unique homologs in humans: IRE1α, IRE1β (structurally more similar to murine IRE1 than human) that is a type 1 of transmembrane protein with two enzymatic features in cytosolic domains, serine/threonine kinase and endoribonuclease (19). IRE1α is ubiquitously expressed, but IRE1β is only in pulmonary and intestinal epithelial and mucosal epithelial tissues. Among the UPR signaling pathways, IRE1α is a key sensor like a switch and rheostat, able to regulate cell fate (43, 44)
RNA GQ structures in cap-independent translation initiation. RNA G-quadruplex (GQ) is a secondary structure that can act as both necessary elements of translation and as translation repressors.1-3 The role of GQ structures in translational modulation depends on the context in which the GQ structure is present.4 However, it is well established that these structures mostly inhibit translation.2,5-7 In fact, rational introduction of GQ structures specifically downregulate the expression of targeted
Objective #2: Stalling, collision, and ribosome recovery in E. coli protein translation. It has long been known that when a ribosome encounters clusters of rare codons, the ribosome can be “rescued,” where a specialized tmRNA molecule allows the ribosome to abort translation of the current peptide chain via the simultaneous completion of the polypeptide with a fast-degradation tag and cleavage/degradation of the mRNA molecule at the site of stalling69-71. Ribosome rescue is a fundamental process that
The smooth ER does not have any ribosomes on it's membrane. It's functions can vary depending on the differences of the cell it is located in. The smooth ER makes lipids, breaks down carbohydrates, and detoxifies substances that may be harmful to the cell. The rough ER appears rough on a microscope because it's surface is covered with small ribosomes. The ribosomes synthesize proteins, and the membrane keeps those proteins separate from the rest of the cell. In addition to making and storing proteins
This includes “Transcription” and then “Translation”. Protein synthesis occurs outside of the nucleus on ribosome with the cytoplasm. The molecules of the “RNA” carries a transcribed genetic message from the DNA to the ribosome, whereas the other molecules of the “RNA” function in the assemble of the protein. RNA is a second type of nuclei acid. The RNA differs from the DNA; this is when it has the base of Uracil
organelles in the cell can be different from each other depending on the type of cell. Plant cells and animal cells both have a cytoskeleton, endoplasmic reticulum (smooth and rough), golgi apparatus, mitochondrion, nucleus, plasma membrane, and ribosomes. Animal and plant cells also have vacuoles, but they are rarely found in animal cells. Vacuoles in animal cells are very small compared to the vacuoles in plant cells. Lysosomes are also rarely found in plant cells but mostly found in animal cells
multi-cellular. All eukaryotic cells have a nucleus, genetic material, a plasma membrane, ribosomes and a cytoplasm. They also have membrane-bound structures called organelles. Eukaryotic