Rna Essay : Rna Self Splicing

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With two T7 promoters, the PCR product will be transcribed by the T7 polymerase. Therefore, a double stranded RNA (dsRNA) product is produced from the plasmid, once IPTG is added to activate the lac operator of T7 polymerase.

Prior to the determination of the primary structure, the RNA strand undergoes splicing which alters the sequence. During splicing introns are removed and exons are randomly re-arranged in a random order. There is 2 problems with this model of attempting to estimate the protein a sequence will form from RNA. Firstly depending on the place of the body at which splicing is occurring, different introns will be removed and also secondly, we need to know which random order the exons will shuffle themselves into.

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coli are picked and the plasmids are purified. The purified plasmids are used as a template for the sequencing reaction. The objective of the lab was to learn how to use the polymerase chain reaction (PCR) to amplify the small subunit ribosomal RNA gene from a bacteria colony, also be able to run an agarose gel to visualize the resulting PCR amplifications and extract the amplified DNA from the agarose gel.

To develop nursing career in an environment that offers tremendous potential for professional growth and achievement; exploring opportunities offered with impactful challenges utilizing my experience, skills and passion.

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