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- 1. Define and Describe aseptic technique in blood collection. 2. Explain the action of SPS as anticoagulant for blood culture.Serum and vitamin extract càn be sterilized using the which are of different sizes.1. Why is it necessary to avoid underfilled blood samples in anticoagulant evacuated tubes? 2. Explain the purpose of thixotropic gel in a blood collection tube. 3. What is the rationale behind the sequence of the order of draw? a. Sterile tube (blood culture) b. Blue-top coagulation tube C. Serum tube (glass) with no additive d. Serum tube with clot activator, with or without е. Heparin tube with or without gel plasma separator f. EDTA tube g. Glycolytic inhibitor tube 4. What are the potentially affected tests if an evacuate tube with silica clot activator was filled first before a blue-top coagulation tube?
- 1. define the latex agglutination (LA) and gelatin particle agglutination tests (GPAT) techniques 2. State the principle underlying the above techniques 3. state the types or forms of the techniques if present1. cite the differences between tube dilution and microtiter plate dilution. 2. why is it important to isolate the peripheral blood mononuclear cells completely from the other cellular components of the blood? 3. what laboratory assays can be performed to assess the efficiency of the sample preparation and isolation of the PBMC?3. Draw and explain the procedure for staining a blood smear.
- 3. Serological reactions in virology. Neutralization test (NT), hemagglutination inhibition test (HIT).38. I- The proper way of transferring a blood sample to an evacuated tube is to push the plunger forcefully to expel the last drop from the syringe. Il- When a blue top is wet, wipe off the inner lining of the tube to avoid contamnination and falsely elevated coagulation studies results. O A. If First statement is CORRECT and second is WRONG O B. If First statement is WRONG and second is CORRECT O C. If BOTH statements are CORRECT O D. If BOTH statements are WRONG 39.1- In the order of draw, tubes for testing glucose levels are filled first prior to tubes used for testing cholesterol levels. Il- When selecting a vein for venipuncture, the basilic vein is usually the vein of choice for obese patients. O A. If First statement is CORRECT and second is WRONG O B. If First statement is WRONG and second is CORRECT O C. If BOTH statements are CORRECT O D. If BOTH statements are WRONG1. Describe the three different type of hemolysis that are observed on blood agar.2. What is a selective medium?3. What is a differential medium?4. Which media can be used to isolate E. coli samples from contaminated lettuce?5. Which two media would be used to identify a sample taken from a patient with suspected gonorrhea?6. Would you be able to grow a sample obtained from a patient's wound (suspected to be infected with MRSA) on EMB? Explain.7. What is the color or TSI for Salmonella?8. What is a fastidious organism?
- 1. Differentiate the 2 specific types of VDRL namely: 1.1 quantitative VDRL 1.2 qualitative VDRL 2. Describe the principle behind the RPR (Rapid Plasma Reagin) Test. 3. What precautionary measures should be observed in the collection and preparation of specimen for VDRL examination?1. Differentiate the tubed media. 2. Discuss the different streaking methods. 3. What is the streaking method used for Antimicrobial Susceptibility testing? 4. How will you determine if a culture is pure or mixed?1. Differentiate the 2 types of antibiotics based on action. Name three examples each. 2. Discuss the different types of antibiotic based chemical composition. Give examples for each. 3. Enumerate 3 qualities of a good chemotherapeutic agent. 4. What are the different methods of sensitivity testing? Discuss briefly.