A mixture of lipids containing phosphatidic acid, cholesterol, testosterone, phosphatidylserine, and phosphatidylethanolamine was applied to a hydrophobic interaction chromatography column. The column was washed with a high salt buffer, and the lipids were then eluted with decreasing salt concentrations. In what order would the lipids be eluted from the column? Explain your answer

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A mixture of lipids containing phosphatidic acid, cholesterol, testosterone, phosphatidylserine, and
phosphatidylethanolamine was applied to a hydrophobic interaction chromatography column. The
column was washed with a high salt buffer, and the lipids were then eluted with decreasing salt
concentrations. In what order would the lipids be eluted from the column? Explain your answer.

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