Assume that you have an “X" solution that you do not know its concentration. But you have other X solutions with known concentrations and you know X solution absorbs maximum light at 575 nm. To calculate the unknown solution concentration, you have done some spectrophotometric obtained data given below. measurements and
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- 3. The following absorbance spectrum was collected. What color is the sample solution? Explain using the color wheel. 1.4- 1.2 0.8 0.6+ 0.4 0.2 300 400 500 600 700 800 wavelength (nm) 4. Given the Beer's Law Plot for permanganate in the Introduction of the lab handout, what would be the concentration of an unknown permanganate sample if the absorbance was 0.308. Absorbance2. Absorbance Data Table Absorbance Concentration 0 mg/dL = blank %3D 0.09 Oi17 0.28 25 mg/dL 50 mg/dL 75 mg/dL 100 mg/dL 035 Unknown solution 6.14 3. Attach a graph of your standard curve. Show the point for the unknown solution on your graph, and determine the concentration of the unknown. Enter this value below (include correct units). Concentration of the unknown solution2. Imagine you have mixed the following known concentrations of a solution. You then measured their absorbance in the spectrophotometer. Construct a standard curve (a line) from the following data points. Use the graph below. Each axis must be labeled with the variable and units, and the scale must be evenly spread. Tube Known concentration (M) Absorbance (AU) 1 0. 0.1 0.03 0.5 0.06 1.0 0.08 5 5.0 0.28 Eseel 22
- Guanosine (C10H13N5O5) in solution has a maximum absorbance at a wavelength of 275 nm. The molar extinction co-efficient at this wavelength is 84M−1cm−1and the path length is 24.7 cm. Through the use of a spectrophotometer, it is found that the that A275= 1.48. What is the concentration of the guanosine solution in grams/litre? Molecular weights (g/mol): C-12, H-1, N-14, O-16 Select one: A. 0.201869 g/L B. 0.435188 g/L C. 0.059919 g/L D. 0.294046 g/L E. 0.000713 g/LHow do you measure the amount of µmoles of a substance from absorbance? If the absorbance is 0.019, the extinction coefficient is 0.0106(µmol/mL)-1 cm-1, path length is 1cm, and the total volume was 4 mL.You record the absorbance of your unknown BSA sample at 0.276. What is the concentration in ug/mL of the unknown assuming you have a standard curve with the line of best fit with y=0.0001x+0.0038 as its equation
- You have carried out an experiment using the spectrophotometry concept. A solutioncontaining compound X is mixed with reagent 1 and then reagent 2. This mixture produces ablue colour whose absorbance (A) could be read at 550 nm. The results are shown below. If the standard solution (compound X) used have a concentration of 1 mM:1. Calculate the quantity of the standard compound X in μmoles for each test tube (1-7).46 50 52 60 41 46 55 Find the ff. WITH SOLUTION PLEASE MeanMedianModeSDVarianceCVQuartile 2Decile 7Percentile 25Percentile 30The column efficiency is constant for a column that uses a cons tant eluent composition. What does this say about the peak width of slowly eluting compounds relative to those of rapidly eluting compounds?
- Why are quantitative absorbance measurements made at lambda max?6. The Worthington's manual reports two extinction coefficients for lysozyme. Corvert one into the other. What is the absorbance at 280 nm of a 0.1 mg/mL lysozyme solution? Use both coefficients to estimate the resultTable 2. Volume of BSA, protein content, and absorbance readings of reference solutions Solution Volume of BSA standard solution (μL) Protein content(μg/mL) Absorbance value At 595 nm 1 0 0 0 2 10 1 0.022 3 30 3 0.065 4 50 5 0.106 5 70 7 0.178 6 100 10 0.299 7 120 12 0.380 Make a graph by plotting the absorbance values versus the BSA protein content (in μg) for theseven reference solutions. When constructing the graph, be…