What cannot be determined using the details of Gram staining process? Question 7 options: bacterial shape cell wall structure antibiotic treatment motility structure

Gram staining technique is the technique which helps to differentiate between gram positive and gram…

Answered: cannot be determined using the details…

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing

Problem 2RQ

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Based from the results obtained from Gram staining, explain why bacterial cells retained or changed their color. Briefly explain the principle behind the procedure. Cite advantages of differential staining method (Gram staining and endospore staining) over simple staining only. List other staining methods aside from those you performed in the laboratory. conclusion:
When performing a Gram stain on a Gram-negative bacteria, why does the bacteria stain pink? Crystal violet stain binds to iodine and remains in the bacterial cell wall because of the thick peptidoglycan layer. Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink. The alcohol wash removes crystal violet and iodine from the bacteria. Crystal violet stain binds to iodine and remains in the bacteria cell wall because of the thick peptidoglycan layer AND Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink. Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink AND The alcohol wash removes crystal violet and iodine from the bacteria.
Which cellular structure is being detected in the motility test? bacterial ribosomes bacterial cilia gram negative cell wall bacterial flagella gram positive cell wall
For the picture below, answer the following questions: A. What is the shape of the bacteria? B. What is the arrangement of the bacteria? C. If this slide shows a Gram stain, what is the Gram stain reaction? If this slide does not show a Gram stain, please state that as your answer. ASM MicrobeLibrary.org Smith
When you interpret a Gram-stained smear, you should also describe the morphology (shape) of the cells, and their arrangement. In the figure below, there are two distinct types of bacteria, distinguishable by Gram stain reaction, and also by their shape and arrangement. Below, describe these characteristics for both bacteria: Gram positive bacterium Gram negative bacterium Morphology cocci bacillus Arrangement
Janet is doing a Gram stain on a mixed culture that is known to contain both Gram positive and Gram negative bacteria. After finishing, she realizes she mixed up the safranin and alcohol steps. What is likely to be observed when she views the slide under the microscope? All cells will appear purple, regardless of whether they are actually Gram negative or Gram positive. All cells will appear pink, regardless of whether they are actually Gram negative or Gram positive. Gram negative cells will be clear, Gram positive cells will be purple. Gram positive cells will be clear, Gram negative cells will be pink. Cells will appear clear, regardless of whether they are actually Gram negative or Gram positive.
Identify the cellular morphology for the above Gram stain. What is the Gram reaction? What is the bacterial shape? What is the bacterial arrangement? J [Choose ] [Choose ] Singles / no arrangement Bacilli Chains Gram-negative Grapelike clusters Gram-positive Spirilla Cocci
Before viewing a specimen of pigmented bacteria on a slide under the light microscope, which of the following usually needs to happen (and why)? Staining (to increase magnification). Heat, radiation or antimicrobial chemical treatment (to kill the bacteria for safe observation). Add immersion oil (to increase resolution). Add a cover slip (to reduce contamination). The viewing chamber needs to be flushed of air (to create a vacuum).
Complete the table below. What color would the bacterial cells have when viewed microscopically after the following treatments: Ammonium Oxalate Crystal Violet Gram Reaction Gram lodine Ethyl Alcohol Safranin Gram-positive Gram-negative
What are the structural differences between Gram-positive and Gram-negative bacteria cell walls? Can you explain what would happen to the cell wall's physical integrity while applying the Gram staining technique?
An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and methylene blue. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following could explain why only blue cells were present? No acid-fast bacteria are present on the slide Methylene blue was not used Carbolfuchsin was not used Acid-alcohol was not used
You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look under the microscope, what color will a Gram positive bacteria, like Staphylococcus aureus, be? Red/Pink Purple colorless

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	antibiotic treatment
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