Ethidium Bromide is a chemical reagent that has been used to detect the presence of nucleic acids. It is known to fluoresce under UV light and the intensity of the fluoresence increases when it binds to the nucleic acids. Ethidium Bromide binds to polynucleotides  a   by binding directly to the bases on one of the strands. b   by intercalating itself between the base pair stacks. c   through the negatively charged phosphate groups. d   through the hydroxyl group of the sugar molecule.

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