1. Explain how scientists could have identified the T toxin 2. Name one enzyme which would have been used during cloning at stage 2. 2.Bacillus thuringiensis (Bt) is a bacterium that lives naturally in the soil. Bt produces a toxic proteinknown as T toxin which is poisonous to caterpillars and some other insects. Genetic engineers haveinserted the gene for the production of T toxin into tomato plants. An outline of the procedurewhich they used is given below.|Identify and cloneT toxin genetransfer gene into callusof tomato cellsDNA extractedfrom Bacillusthuringiensistomato plant containingcells capable ofproducing T toxingrow cells which havethe T toxin gene intotomato plants

Bacillus thuringiensis is a bacterium and now used as genetically modified organism to grow the pest…

Answered: Explain how scientists could have…

Explain how scientists could have identified the T toxin 2. Name one enzyme which would have been used during cloning at stage 2.

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications

Section: Chapter Questions

Problem 6TYK

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Concept explainers

Genetic Recombination

Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.

Microbial Genetics

Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.

Question

1. Explain how scientists could have identified the T toxin

2. Name one enzyme which would have been used during cloning at stage 2.

2.
Bacillus thuringiensis (Bt) is a bacterium that lives naturally in the soil. Bt produces a toxic protein
known as T toxin which is poisonous to caterpillars and some other insects. Genetic engineers have
inserted the gene for the production of T toxin into tomato plants. An outline of the procedure
which they used is given below.
|Identify and clone
T toxin gene
transfer gene into callus
of tomato cells
DNA extracted
from Bacillus
thuringiensis
tomato plant containing
cells capable of
producing T toxin
grow cells which have
the T toxin gene into
tomato plants

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