indicators, key used for/ determines reagents, or key positive result ingredients |1. crystal violet Comments/ Medium/test negative result additional info decolorization is key. Must gram reaction and size and 2. iodine morfdant Gram stain stains purple stains pink include +/- controls on 3. gram's declorizer 4. safranin shape every slide to verify result quadrant streak- isolation of pure culture of bacteria check this for any pigmentation of colonies TSA Eosin methylene blue agar (EMB) 1. no blackening of medium 2.same color of added multi test medium (3 results reagent (not red) 3. stab is visible an defined with growth 1. blackening of medium 2. red color in added 1. sulfur/H2S 2. indole/tryptophasnase 3. motility/flagella 1. ferrous salts 2. Kovac's 3. low % agarose reagent 3. fuzzy appearance of medium/stab not visible SIM in one medium) motility agar Brewer's plate in Anaerobe jar Fluid thioglycollate (FTM)

indicators, key used for/ determines reagents, or key positive result ingredients |1. crystal violet Comments/ Medium/test negative result additional info decolorization is key. Must gram reaction and size and 2. iodine morfdant Gram stain stains purple stains pink include +/- controls on 3. gram's declorizer 4. safranin shape every slide to verify result quadrant streak- isolation of pure culture of bacteria check this for any pigmentation of colonies TSA Eosin methylene blue agar (EMB) 1. no blackening of medium 2.same color of added multi test medium (3 results reagent (not red) 3. stab is visible an defined with growth 1. blackening of medium 2. red color in added 1. sulfur/H2S 2. indole/tryptophasnase 3. motility/flagella 1. ferrous salts 2. Kovac's 3. low % agarose reagent 3. fuzzy appearance of medium/stab not visible SIM in one medium) motility agar Brewer's plate in Anaerobe jar Fluid thioglycollate (FTM)

Principles Of Pharmacology Med Assist

6th Edition

ISBN:9781337512442

Author:RICE

Publisher:RICE

Chapter15: Antibiotic Agents

Section: Chapter Questions

Problem 20RQ

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Answer TRUE OR FALSE only. 1. All types or kinds of media powder has a standard ratio of 23:1000. Meaning that 1000 ml of water is needed to dissolve 23 grams of powder medium. 2. Simple stains use one dye and cannot differentiate various types of bacteria while differential stains use several dyes in order to differentiate between different kinds of bacteria. 3. A certain student prepared his fixed sample for examination by pouring Nigrosin which is an acidic stain. He then observed that under the microscope the background was stained but the bacterial cells were untouched. This student therefore adapted the acid-fast staining procedure. 4. In doing serial dilutions, the original sample must be shaken at least 25 times to obtain a uniform distribution of organisms. 5. Label all reagents with its name, concentration and date of its preparation except for water.
mead. Post Lab #2 Smear & Stain Preparation Name: Leidiawa MontaNo Date: 1. Why are thick or dense smears less likely to provide a good smear preparation for microscopic evaluation? Please explain. Becapse, it will diminish the amount ds latcan Pass through by mam 1t difficult to See under the micioscol e, s less liket to Prowde a go image. 2. What could potentially happen if you leave the slide exposed for too long to the open flame? Why do you have to be careful? we can form ring Patterns if we expose the slide for ta0 the flame 3. During the preparation of a smear leading into simple staining of the bacterial culture S. epidermidis you forgot to heat fix the slide. What would you see on this slide as compared to a slide that was properly prepared? Please explain. 4. You partner stained bacterial cells and saw only the background and not the actual cell was stained. Your partner thought this was a mistake. Please explain what type of staining method this is, how it works and why the…
G When is it appropriate for gloves X + rface/defaultui/player/modern.html?configuration=&preventRightClick=False&cc=en-US&ieCompatibilityMod... 2 Exit rientation to Infection Prevention Which of the following are proper disinfection steps? Check all the apply. O Remove organic matter O Disinfect only O Follow suggested contact time O Mix cleaning products to make the disinfectant more powerful O Disinfect O Disregard the product labels SUBMIT ENG IN
HonorSociety org č. * SpiasiLa * Maps New Tab Describe your color observations of the Nitrate test. a. after adding Reagents A, B (and/or znic): cements b. Did the organism reduce nitrate? (yes or no) ments c. Is the final product nitrate, nitrite or ammonia/nitrogen gas? sions ing es Question 13 4 pts y Resources ules Based on your observations of the SIM test: ple a. Did sulfur reduction occur (yes/no)? zes b. Did the organism produce the enzyme tryptophanase (yes/no)? abus m c. Is the organism positive or negative for the motility test?
entic X G Which of the following are prope X elnterface/defaultui/player/modern.html?configuration=&preventRightClick=False&cc=en-US&ieCompatibilityMod.. 2 Orientation to Infection Prevention Exit Which of the following are proper disinfection steps? Check all the apply. O Remove organic matter O Disinfect only O Follow suggested contact time O Mix cleaning products to make the disinfectant more powerful O Disinfect O Disregard the product labels SUBMIT ENG 令中) IN 40 ho
Idenitfy the statements whether they are TRUE or FALSE 1. You should use P-20 micropipettes to transfer volumes for spread plating. 2. You should use P-200 micropipettes to transfer volumes for pour plating.
of 1 stion s page 30- F2 Which of the following terms is associated with the probability of a preparation not being sterile after it has been exposed to a sterilization process? Select one: Oa. Particulate Matter level Qb. Sterility Assurance level О с. Pyrogen Load level O d. Optimal Sterilization level F3 F4 F5 Ol پیار Q Search F6 F7 F8 F9 === 8 9 F10 Time left 22:34:03 F11 Next page
Cause-and-Effect Analysis: Given the conditions below, describe the effect on the staining procedure and explain briefly: 1. Excessive heat was applied during fixation 2. Low concentration of crystal violet used during gram staining 3. Excessive washing between steps 4. Insufficient decolorization 5. Excessive counterstaining
Observations and Results ml of Dilution Plated Final Dilution on Plate Number of Colonies Bacterial Count per ml of Sample (CFU/ml) Average Count per ml of Sample (CFU/ml) Dilution Factor Plate 1A H| 1:10 Iml 18 :100 Iml Iml 28 H41:10000Iml 3A H5:100.000iml 10 10 100 1000 103 10000104 100000 105 2A H31:1000 3B table helpbfor the missing boxes
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Why is it necessary to use a 24-hrold culture in gram staining? What does Gram staining reveal owing to its use in clinical diagnosis? Indicate color staining results of gram positive and negative bacteria for every hypothetical scenario. Modification in Procedure Staining results 1. Crystal violet was replaced with methylene blue. G+ G- 2. Mordant was skipped G+ G- 3. Decolorizing step was omitted G+ G- 4. counterstain was not used? G+ G- 5. Crystal violet and safranin was swapped in position. G+ G- What bacterial specimen represent the following: (a) positive simple staining; and (b) negative simple staining viewed under OIO. Provide details such as specimen and bacterial stain used and reference. Positive Staining 1000 X Negative Staining 1000 X