QUESTIONS IN THE COLUMN CHROMATOGRAPHY PART OF THIS EXPERIMENT: 1. In what order would you have to add the solvents (water and ethanol) to remove methylene blue first and fluorescein second? 2. What would happen if a mixture of ethanol and water were used as eluting solvents? IN THE TLC PART OF THIS EXPERIMENT: 3. What would be the result if too much solution is spotted on the TLC plate? 4. Why must the solution be spotted above the level of the developing solvent in the chamber?

QUESTIONS IN THE COLUMN CHROMATOGRAPHY PART OF THIS EXPERIMENT: 1. In what order would you have to add the solvents (water and ethanol) to remove methylene blue first and fluorescein second? 2. What would happen if a mixture of ethanol and water were used as eluting solvents? IN THE TLC PART OF THIS EXPERIMENT: 3. What would be the result if too much solution is spotted on the TLC plate? 4. Why must the solution be spotted above the level of the developing solvent in the chamber?

EBK A SMALL SCALE APPROACH TO ORGANIC L

4th Edition

ISBN:9781305446021

Author:Lampman

Publisher:Lampman

Chapter89: Thin-layer Chromatography

Section: Chapter Questions

Problem 1P

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