Why it is possible/ applicable to use recrystallization and vacuum filtration with Luminol? Cite some reasons.(Explain in at least 1 paragraph)
Q: Please answer it ASAP and correctly for an Upvote. Thank you. •Following a sterilization process…
A: Sterilization : It is the process physical or chemical which destroys all forms of life , is used…
Q: Explain why only gram-negative cells undergo decolorization during the gram staining procedure.
A: Gram staining is a method in which cells stain either pink (gram-negative) or purple (gram-positive)…
Q: what does chroomatography observation mean
A: Column fractionation was developed in the 1890s as a technique of separating the various components…
Q: Identify the method of sterilization/ chemical agent described At 15psi, the temperature reaches…
A: Given information: At 15psi, the temperature reaches 121 degree Celsius requiring only 15 to 20…
Q: Find the amount of dehydrated medium needed to prepare 15 BUTTS, 20 SLANTS, and 25 BUTT-SLANTS.
A: Slants, Butt-slants, and butts are different types of media prepared for bacterial growth. Slants…
Q: In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside…
A: Several laboratory techniques necessitate smear preparation. A smear can be made using either broth…
Q: Why are products mixed with phenols and surfactants preferred when cleaning surfaces? Question 14…
A: Disinfection is the procedure by which various bacteria, microbes can be killed by using different…
Q: eservatives cannot be used for direct + mount ?
A: The disease is a state of the body in which the physiological mechanisms do not function in a normal…
Q: _____ kills pathogens and some spoilage organisms but does not sterilize substances. Tyndallization…
A: Sterilization refers to any process that removes, kills, or deactivates all forms of life, in…
Q: Microbiology
A: Gram staining is a technique which is used to identify the bacterial cell type, whether it is gram…
Q: FOOD MICROSCOPY: STARCH IDENTIFICATION The following figures are the photomicrographs of the…
A: Starch is mainly found in a plant source, as starch is a storage material.
Q: Serial dilution is recommended prior to pour and spread plate. Why is this so?
A: Pour Plate: it is a technique in which A plate made by combining the inoculum which is cooled but…
Q: What will pose an ethical issue in these procedures are performed - sterilization?
A: Sterilization is a permanent method of family planning. It is one of the most common method of…
Q: Match the expected result (purple, red, or colorless) to the following descriptions of Gram-stained…
A: Gram staining is a common staining technique that is used to differentiate bacteria based on their…
Q: Enumerate the step-by-step procedures of performing both techniques using a schematic diagram. -…
A:
Q: All recipes are given per litre unless specified. Lambda Broth (LB) Plates…
A: Give, The final volume (V2) = 5mL. The stock concentration of MgSO4 (C1)= 1M The desired…
Q: Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can…
A: According to the guidelines we have to answer first 2-3 question rest you can ask separately thank…
Q: discuss the clinical significance, principles, reagents (if any) and positive and negative results…
A: *Note: Since you have posted a question with multiple sub-parts, we will solve first three sub-parts…
Q: What is the purpose of the quadrant streak plate method? - How could this method be used in a…
A: In quadrant streak plate method, first of all the plate on which bacteria has to be grown that is on…
Q: Which of the following can most likely increase the yield of recrystallized benzoic acid? CHOICES:…
A: Recrystallization is a method that is used for the purification of solid compounds by the removal of…
Q: Indicate color staining results of gram positive and negative bacteria for every hypothetical…
A: there are two group of bacteria- Gram-positive and gram Negative this technique is based on the…
Q: asystole
A: The cardiac cycle is the performance of the human heart from the beginning of one heartbeat to the…
Q: Prove if the statement is TRUE or False with complete solution. At a dilution factor of,…
A: Data on colonies grown after plating pasteurised milk samples is provided. Because each cell…
Q: You are testing unpasteurized milk for the presence of bacterial contamination. Starting from the…
A:
Q: Provide three reasons why the use of aseptic technique is essential when handling microbial cultures…
A: Aseptic techniques means using practices and procedures to prevent contamination from pathogens. It…
Q: The following figures are the photomicrographs of the samples taken with Kern&Sohn Camera Microscope…
A: Ans: Lugols solution: The Lugols solution is 2.5 to 25% weight by volume total iodine.
Q: I need hand written solution
A: Global oxygen delivery (DO2) is the total amount of oxygen delivered to the tissues per minute,…
Q: What are the advantages and disadvantages of using the Wet Mount technique?
A: As per bartleby guidelines, we are suppose to answer only one question kindly post other separately.…
Q: For preparation of a smear on a slide, what is the purpose of heat fixation? What problems can arise…
A: The preparing a slide that has to be observed under a microscope there are multiple steps that are…
Q: Give the drug examples of emulsion prepare by these method:- 1)dry gum method 2)wet gum method…
A: Emulsions are heterogeneous biphasic systems. Thus, there are two immiscible phases. One of the…
Q: Are TSA, MRS and MOX differential media? Why or why not?
A: In microbial biology, differential media refers to any growth media that comprises of certain…
Q: Why is it necessary to perform a plate count in conjunction with the turbidimetry procedure?
A: Plate counting is one of the most fundamental techniques used in microbiology. It is used to…
Q: All the following products must undergo sterility testing EXCEPT: Oral tablets Injections…
A: The healthcare industry uses various medical equipment and instruments that aid in the treatment of…
Q: In performing the Kirby-Bauer procedure in a clinical laboratory setting, why must the agar be a…
A: The Kirby Bauer antibiotic test, also known as the disc-diffusion test, employs an antibiotic disc…
Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…
A: The staining is done to enhance the visualization of cell and certain cellular component under a…
Q: Outline in detail the procedure for celloidin embedding .
A: Embedding is that the method during which the tissues or the specimens are capsulate in a very mass…
Q: Write the protocol for lactophenol blue stain method indicating; materials, reagents, Principle,…
A: Fungi consist of a broad class of organisms from single yeast to multicellular organisms like…
Q: Write the instructions, reagents, principles and the procedures for the following. 1. Periodic acid…
A: (AS PER OUR GUIDELINES WE ARE PROVIDING YOU ANSWER OF 1ST TWO QUESTIONS. YOU CAN POST OTHER…
Q: The figure above depicts an agar cube with a side length of 13\, \text{mm}13mm13, start text, m, m,…
A: The interior, unpenetrated region of the agar cube is also cube-shaped because the orange dye…
Q: Which of the following is/are true regarding the acid-fast stain? (There may be more than one…
A: Answer First option Non-acid-fast microbes appear blue in a completed acid-fast stain.
Q: Sulfamethoxazole is a type of sulfonamide antibiotic. Its absorbance ranges from 250-300 nm in a UV…
A: Sulfamethoxazole Sulfamethoxazole is a sulfonamide based bacteriostatic antibiotic, widely used to…
Q: The acid-fast bacterium, Mycobacterium smegmatis, is relatively safe to be used by students in…
A: Acid fast bacteria are those which responds to acid fast staining . Most of the members of…
Q: Alternative name for Moist-heat sterilization Select one: Oven sterilization Autoclaving Ionizing…
A: Sterilization is any technique that eliminates, removes, or disengages every living organism…
Q: Which is more Suitable for long-term storage: agar slant or plate? Explain why?
A: In order to isolate the bacteria from samples, they need to be inoculated into a growth medium which…
Q: In preparing bacterial smear, why do we need to pass the slide over the flame?
A: since this is a multi-part question, so I am answering only 1st part. please upload other questions…
Q: Explain each type of anti-fouling system (application and procedure) Electrolytic system Chemical…
A: Anti fouling is a coating which can be done on the hull of any ship It prevents the overgrowth of…
Q: Why should agar media be completely dissolved before they are dispensed in tubes and plates? What…
A: Introduction A growth medium, also known as a culture medium, is a solid, liquid, or semi-solid…
Q: What is the hazard of the splattering tendency in flame sterilizing an inoculating loop? Can…
A: Aseptic preparation of culture media :-
Q: To dilute a bacterial culture, 500 μl of a 16 hour culture is mixed with fresh culture media to a…
A: Given Values: Volume of the 16 hour culture = 500 μl Final Volume of the culture media = 5 ml
Q: what practical importance are air borne microorganisms to the laboratory workers? What precautions…
A: Laboratories are the areas where lot of experiments and research is performed. It deals with lot of…
Step by step
Solved in 2 steps
- What is the physical appearance (solid, liquid, semi-solid) of following media: agar plateIn sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down. For "autoclaving" For Dry heat oven Can be sterilized only sterilization with either Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-ml graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-ml beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperSolid and liquid media are commonly prepared based on manufacturer’s instructions. If no instruction is provided on how the media should be sterilized, what would be the best option? METHOD:RATIONALE:
- What is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tube3) Define gel electrophoresis, including its theory and application. Describe the steps of running gel electrophoresis using the following image. More detailed reading: https://www.sciencedirect.com/topics/medicine-and-dentistry/agar-gel-electrophoresis POWER SUPPLY CATHODE ELECTROPHORETIC BUFFER ANODE WELL SAMPLE AGAROSE GEL POWER SUPPLY CATHODE ANOCE HIGH MOLECULAR WEIGHT SPECIES LOW MOLECULAR WEIGHT ANALYTESWrite the protocol for lactophenol blue stain method indicating; materials, reagents, Principle, procedure and results.
- Answer the following questions: 1. Why do we pass the smear in the open flame of the bunsen burner 3 times? What do we want to achieve by doing this? 2. Is the size of the bacterial smear on the slide very critical to the analysis? Why or why not? 3. Is the thickness of the bacterial smear on the slide very critical to the analysis? Why or why not? 4. Why do we pass the smear in a gentle stream of water after flooding it with a stain? What do we want to prevent by doing this? 5. Is the duration or time of stain application very critical in simple staining? Why or why not? NOTE: Please try to answer all of the questions asked, i promise to give you a good ratingsWhich of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.Explain the importance of steam during the acid-fast staining procedure. Discuss the advantages of differential staining procedures over the simple staining techniques. List and briefly describe the functions/purpose of each of the different stains used in acid fast staining.
- Why is it best to use sterile distilled water in the preparation of microbial suspension and dry smears for slide preparation? What possible error could happen if a glass slide is reused for slide preparation?Explain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a dayEMB agar inhibits the growth of Gram-positive bacteria while encouraging the growth of Gram-negative bacteria. It also can determine if some bacteria use lactose. It is particularly effective at determining if E. coli is present in a specimen. Define what type(s) of media EMB agar is, using as many terms in the answer list as are applicable.