You isolated a gene expressed in differentiated neurons in mice. You then fused various fragments of the gene (shown as dark lines in the following figure) to a GFP reporter lacking either enhancers or a promoter. The resulting clones were introduced into neurons in tissue culture, and the level of GFP expression was monitored by looking for green fluorescence. From the results that follow, which region contains - the promoter and a neuronal enhancer? Regulatory region of nerve gene GFP GFP expression Fragments fused to GFP 80 ab c d e 19

Human Anatomy & Physiology (11th Edition)
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Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Problem 19.10
You isolated a gene expressed in differentiated neurons in mice. You then fused various
fragments of the gene (shown as dark lines in the following figure) to a GFP reporter
lacking either enhancers or a promoter. The resulting clones were introduced into
neurons in tissue culture, and the level of GFP expression was monitored by looking for
green fluorescence. From the results that follow, which region contains
- the promoter and
- a neuronal enhancer?
Regulatory region of nerve gene
GFP
GFP expression Frågments fused to GFP
80
80
ab c
d e
19
Transcribed Image Text:Problem 19.10 You isolated a gene expressed in differentiated neurons in mice. You then fused various fragments of the gene (shown as dark lines in the following figure) to a GFP reporter lacking either enhancers or a promoter. The resulting clones were introduced into neurons in tissue culture, and the level of GFP expression was monitored by looking for green fluorescence. From the results that follow, which region contains - the promoter and - a neuronal enhancer? Regulatory region of nerve gene GFP GFP expression Frågments fused to GFP 80 80 ab c d e 19
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