Microbiology: A Systems Approach
5th Edition
ISBN: 9781259706615
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
expand_more
expand_more
format_list_bulleted
Concept explainers
Textbook Question
Chapter 10, Problem 4CTQ
a. Define the term RFLP. Explain how RFLPs are created and why they are useful in DNA analysis.
b. In the following DNA profile, identify the pathogen that is making the two patients ill, and explain your answer.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
Explain how PCR/OLA (polymerase chain reaction/oligonucleotide ligation assay) can be used in the diagnosis of sickle cell disorder . Would you recommend this method for routine diagnosis of sickle cell disorder? Explain
What does the acronym TPA stand for and how is TPA used in diagnostic medicine? Explain briefly.
Write and discuss about the analysis procedure used for carrier screening for the single-gene diseases that is routinely conducted in different countries. (Subject: Genetic engineering).
Chapter 10 Solutions
Microbiology: A Systems Approach
Ch. 10.1 - Provide examples of practical applications of...Ch. 10.2 - Prob. 2AYPCh. 10.2 - Describe how gel electrophoresis is used to...Ch. 10.2 - Prob. 4AYPCh. 10.2 - Prob. 5AYPCh. 10.3 - Prob. 6AYPCh. 10.3 - List examples of genetically modified bacteria,...Ch. 10.4 - Prob. 8AYPCh. 10.4 - Prob. 9AYPCh. 10.5 - Outline in general terms the process of DNA...
Ch. 10.5 - Prob. 11AYPCh. 10.5 - Prob. 12AYPCh. 10.5 - Prob. 13AYPCh. 10.6 - Prob. 14AYPCh. 10 - Which of the following is/are not essential to...Ch. 10 - Prob. 2MCQCh. 10 - The function of ligase is to a. rejoin segments of...Ch. 10 - The creation of biological molecules entirely from...Ch. 10 - Which of the following sequences, when combined...Ch. 10 - A region of DNA in a plasmid that is recognized by...Ch. 10 - Prob. 7MCQCh. 10 - Which of the following is a primary participant in...Ch. 10 - Single nucleotide polymorphisms are found in a....Ch. 10 - Microarrays are used to monitor a. the rate of DNA...Ch. 10 - Prob. 11TFCh. 10 - A nucleic acid probe can be used to identify...Ch. 10 - Prob. 13TFCh. 10 - In order to detect recombinant cells, plasmids...Ch. 10 - Plasmids are the only vectors currently available...Ch. 10 - You are a public health official trying to...Ch. 10 - a.Construct a strand of complementary DNA (cDNA)...Ch. 10 - a.Explain whether or not DNA polymerase from a...Ch. 10 - a.Define the term RFLP. Explain how RFLPs are...Ch. 10 - Prob. 5CTQCh. 10 - From chapter 6, figure 6.19. What has happened to...Ch. 10 - Prob. 2VCCh. 10 - Using the words that follow, please create a...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- introduction Background information about the TLC technique ,should include: a- The phases. b- The principle of the technique. 2- Basic structure of amino acids. 3- Definition of the Retention Factor (Rf). aim :arrow_forward1.) Chronic Myelogenous Leukemia (CML)-Briefly describe the disease and provide the most common symptoms/features of the disease.-Describe the cause/s of the disease.-Using a diagram, outline the processes of the best DNA technology tools/techniques involved in the diagnosis of the disease.-Present the best therapeutic DNA technology tool/technique for the disease by describing the tool and discussing the procedure.-Identify the challenges or issues that a scientist may face for using the DNA technology diagnostic and therapeutic tools and applications you have presented.arrow_forwardProvide a brief summary (3 – 5 sentences) about information of the GATA3 gene (frequency of mutations, types of mutations, details of patient data such as health symptoms expressed due to the mutated gene above , etc.).arrow_forward
- I understand that microarrays are being used to define the molecular abnormality and the prognosis in some patients with leukaemia. What are microarrays?arrow_forwarda. Briefly discuss (using three sentences) how the concepts and/or techniques in molecular biology are applied in COVID-19 pandemic, and: b. For each topic (Covid-19 pandemic), give any issues, benefits, or drawbacks of the application (still using three sentences).arrow_forwardSerological testing:a. Describe the key principles behind serological testing in the identification of an unknown organism.arrow_forward
- A. Prepare a diagram with labels showing the similarities and differences among the four major types of ELISA based on their capture and detection systems. A1. Give at least one clinical application for each type of ELISA. B. What is the purpose of adding horseradish peroxidase, alkaline phosphatase, and their respective substrates?arrow_forwardDiscuss the importance of not coding a procedure directly from the Alphabetic Index. Explain the effect of following the CPT guidelines when choosing codes.arrow_forwardFor the following diseases, describe the best technique for diagnosing them. Please make sure you include how you would tell someone with the disease from someone without the disease. A. Hemochromatosis, a recessive genetic disease caused by mutations in the HFE gene, which helps sense iron levels. Mutations in the HFE gene lead to the absorption and storage of excess iron, damaging the liver, heart, and pancreas. The HFE protein is still produced, but does not function properly. B. Factor V Leiden thrombophilia is caused by a point mutation at position 1691 in exon 10 of the Factor V clotting factor gene that changes an arginine into a glutamine. This change removes one of the cleavage sites for activated protein C and leads to an increased tendency to clot. C. Babesiosis is an infection by the parasite Babesia microti that is carried by deer ticks, similar to Lyme disease. The parasite infects red blood cells in humans.arrow_forward
- Define Blue/White Screening. Explain principle mechanism of this approach briefly.arrow_forwardFor the following diseases, describe the best technique for diagnosing them. Please make sure you include how you would tell someone with the disease from someone without the disease. A. Hemochromatosis, a recessive genetic disease caused by mutations in the HFE gene, which helps sense iron levels. Mutations in the HFE gene lead to the absorption and storage of excess iron, damaging the liver, heart, and pancreas. The HFE protein is still produced, but does not function properly.arrow_forwardUse the following information to answer the next question. Human genes were integrated into the chromosomes of pig sperm using the procedure of sperm-mediated gene transfer (SMGT). In the first successful SMGT procedure, scientists used an enzyme to integrate three human genes into the chromosomes of pig sperm. Approximately 90% of the resulting pig embryos carried all three human genes in very cell. The presence of the human genes in every cell of a pig embryo is evidence that the genes went through Select one: a. mRNA transcription b. DNA replication c. mRNA translation d. DNA transcriptionarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Understanding Health Insurance: A Guide to Billin...Health & NutritionISBN:9781337679480Author:GREENPublisher:Cengage
Understanding Health Insurance: A Guide to Billin...
Health & Nutrition
ISBN:9781337679480
Author:GREEN
Publisher:Cengage
What is cancer? What causes cancer and how is it treated? *UPDATE*; Author: Cancer Treatment Centers of America - CTCA;https://www.youtube.com/watch?v=_N1Sk3aiSCE;License: Standard Youtube License