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A Transgenic Lines

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Three independent homozygous transgenic lines with significantly higher Os08g01480 expression were selected for further characterization. In early stages of vegetative growth on
Soilrite, no phenotypic difference was observed in transgenic lines in comparison to WT.
However, there was clear difference in bolting time between WT and transgenic lines
(Supplementary Fig. S2). The visible changes in early plant growth between WT and transgenic lines were analyzed through measuring root length after growth on ½ MS plates for 11 days. All the transgenic lines showed significantly increased root length compared to
WT plants. To study whether increase in root length was due to early germination, germination rate of WT and transgenic lines was …show more content…

At the same time, significantly lower (~25%, 20%, 40%, and
7%, decrease) root length inhibition in transgenic plants was recorded in comparison to root length of NT transgenic plants. (Fig. 1).
For salt and osmotic stress, WT and transgenic line were grown on NaCl (50 mM) and mannitol (150 mM) for 11 d. Root length of WT showed significant decrease (70%) as compared to transgenic line (30%-50%) during salt stress as compared to NT; WT and transgenic seedlings, whereas in osmotic stress, ~50% and up to 40% decrease was observed in WT and transgenic lines compared to NT; WT and transgenic plants. It is concluded that in WT there was ~40% and 20% more reduction in case of salt stress and osmotic stress respectively (Fig. 2).
For studying effect of cold and heat, after stratification seeds were kept at -20˚C and
37˚C respectively for 4 h and then transferred to control conditions. As much as ~60% and
40% decrease in root length of WT was noted during cold and heat stress, respectively when compared to NT; WT plants, while ~25% and ~15% decrease of root length was recorded in transgenic lines at time of heat and cold stress, respectively when compared to NT transgenic plants. This result indicated ~40% and ~20% reduction in root length of WT compared to transgenic lines subjected to cold and heat stresses (Fig. 3). These studies

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