Three independent homozygous transgenic lines with significantly higher Os08g01480 expression were selected for further characterization. In early stages of vegetative growth on
Soilrite, no phenotypic difference was observed in transgenic lines in comparison to WT.
However, there was clear difference in bolting time between WT and transgenic lines
(Supplementary Fig. S2). The visible changes in early plant growth between WT and transgenic lines were analyzed through measuring root length after growth on ½ MS plates for 11 days. All the transgenic lines showed significantly increased root length compared to
WT plants. To study whether increase in root length was due to early germination, germination rate of WT and transgenic lines was
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At the same time, significantly lower (~25%, 20%, 40%, and
7%, decrease) root length inhibition in transgenic plants was recorded in comparison to root length of NT transgenic plants. (Fig. 1).
For salt and osmotic stress, WT and transgenic line were grown on NaCl (50 mM) and mannitol (150 mM) for 11 d. Root length of WT showed significant decrease (70%) as compared to transgenic line (30%-50%) during salt stress as compared to NT; WT and transgenic seedlings, whereas in osmotic stress, ~50% and up to 40% decrease was observed in WT and transgenic lines compared to NT; WT and transgenic plants. It is concluded that in WT there was ~40% and 20% more reduction in case of salt stress and osmotic stress respectively (Fig. 2).
For studying effect of cold and heat, after stratification seeds were kept at -20˚C and
37˚C respectively for 4 h and then transferred to control conditions. As much as ~60% and
40% decrease in root length of WT was noted during cold and heat stress, respectively when compared to NT; WT plants, while ~25% and ~15% decrease of root length was recorded in transgenic lines at time of heat and cold stress, respectively when compared to NT transgenic plants. This result indicated ~40% and ~20% reduction in root length of WT compared to transgenic lines subjected to cold and heat stresses (Fig. 3). These studies
Throughout this experiment, we are researching the effect on the growth and survival of Wisconsin Fast Plants using fertilizer pellets to help with the growth of the plants. Wisconsin Fast Plants is a plant member of the crucifer family which is related to other plants (vegetables) such as cabbage, broccoli, turnips, etc. This plants are small and can grow very easily because they go through their cell cycle around 40 days. Wisconsin Fast Plants Fertilizers are different materials used that can provide plants with the nutrients it need to grow. (1) These plants are a good model system to study because they grew very quickly and didn’t need a lot of resources to grow making them the perfect plant to use for studies. (4) By using the fertilizers,
might affect the fitness of each variant. In other words which factors might increase plant growth, survival,
This experiment was performed to give a better idea of interspecific competition and intraspecific competition between radish seeds and wheat seeds at high and low densities. By planting two species only pots and two combined species pots our results showed that the radish seeds performed better at both interspecific and intraspecific competitions and concluded that the lower the pot density the more resources and growth. 32 radish seeds were evenly planted in pot A1 followed by 32 wheat seeds planted in pot A2 and 16 of each radish and wheat seeds combined (32 total) planted in pot AB1. We repeated this procedure again but this time planting 96 radish seeds evenly in pot A2, 96 wheat seeds in pot B2 and 48 of each radish and wheat seeds combined (96
Progeny mean of these lines for canopy temperature at booting was 17.74, at heading 20.78, at anthesis 26.7 and at grain filling 29.67 as compared to parent GW322 with 18.2,22.2,26.18,31.96 and 18.2,24.3,28.4,34.5 at booting, heading ,anthesis and grain filling stage under non stress and stress condition respectively which was comparatively less than recurrent parent. For chlorophyll content measured by ,SPAD was ranging from 49.34 to 44 from booting to grain filling stage in progenies and 47.6 to 44 and 43 to 36 in GW322 under non stress and stress condition respectively. Similarly, NDVI, Vegetation greenness index recorded in booting to grain filling stage in progenies was 0.67 to 0.5 when compared to parent with 0.61 to 0.51 under irrigated and 0.54 to 0.36 in rainfed condition which was not found to be affected due to water stress. SPAD values and NDVI was started declining in postanthesis to grain filling stage in all MABB derived improved lines. Staygreen scale of 2.6 obsreved in GW322 in irrigated and 2 under rainfed condition as in case of progenies it was 6. Mean yield per sq. meter was 582.48 (g) and thousand kernel weight 44.84 (g)
In all sources researched prior to starting the experiment, each solution, when used as a soaking solution for plant seeds before planting, was able to somehow help the end results, if it was in germination rate, plant length, etc. But, each solution has different chemical properties that means each solution must soak the seeds for a different amount of time to help germination. And in this
ABSTRACT — In the present investigation, it was revealed that treatment with paclobutrazol (PBZ) @ 2.5, 5.0, 10 and 20 µg mL–1 resulted in shoot length and shoot dry weight to decrease significantly under normal condition, but increased significantly in salinity (4 dSm-1 and 8 dSm-1) with respect to control. Treatment with PBZ resulted in significant increase in root length and root dry weight as compared to control under both normal (PBZ) and saline conditions. Total chlorophyll was recorded to increase significantly at vegetative and flowering stages as compared to control. Hydrogen peroxide decreased significantly at vegetative and flowering stages. Treatment with PBZ resulted in significant increase in protein and total sugar content at
Plants are frequently subjected to a wide range of environmental temperatures that may affect the duration of mitotic stages. We investigated the influence of temperature on the duration of mitotic stages in the onion root tip squashes, Allium cepa, by counting the number of cells appearing in each stage of mitosis when exposed to conditions in room temperature and cold temperature. We found that the average number of cells in interphase increased with the decrease in temperature, and the rate of mitosis increased with an increase in temperature. Thus, the increased amount of cells in interphase compared to the amount of cells in other stages at the cold temperature could be the result of the higher activation energy required in situations of lower temperatures, decreasing the rate of respiration and slowing the process of mitosis.
This experiment shows that contaminants, in this experiment rock salt, does have a direct correlation between plant growth and color over the 4 weeks. As shown in each graph figures, the independent variable is the weeks and the dependent variable is the plant heights. Figures 1 and 2 shows that the untreated water caused the control group plant to have the highest amount of growth. The average heights of all the plants generally increased as the weeks increased. The standard deviation of the plant heights increased as the weeks increased. The color for the radish plant (Raphanus sativus) generally did not change during the experiment. Chlorosis did not take place in plants leaves, but kept the green fill for this experiment. The highest
Auxin initiates the growth of the stem/roots via the Acid Growth Theory, which states that when certain cells come in contact with auxin protons are excreted into to the apoplast at a higher rate than usual, therefore lowering the pH of the apoplast (Rayle and Cleland, 1992). The low pH of the environment initiates the cell wall-loosening process involving the rearrangement of load-bearing bonds within the cell wall which is controlled through specialized proteins known as expansins. When the cell wall is sufficiently loosened water is allowed to enter the cell via osmosis causing the cell to enlarge. The uptake of the water increase internal turgor pressure, which causes the cell membrane to push against the cell wall allowing it to extend. The extension of the cell wall is halted after approximately 30 to 60 minutes via auxin enabling genes which engage other cell elongation methods (Plant & Soil Sciences eLibrary, no
Since many of the plants followed this pattern, the trend between their mount and growth will be calculated using negative numbers for the plants that went down in weight. The plants that were grown in wood’s average growth in weight was -0.363 grams and their growth in leaf length was -0.05 centimeters. For the plants grown in stones, the average growth in weight was approximately -0.327 grams while the average growth in leaf length was 0 centimeters. Lastly, the average weight growth for the control group in a plant hanger was -0.202 grams and their leaf growth was 0 centimeters. Plants 3 (grown in wood) and 4 (grown in stones) exhibited the largest decrease in weight, losing 0.633 and .607 grams from the first to 36th day. The plants with the least decrease in weight were plants 5 (grown in stones) and 7 (control group), with a loss of 0.145 grams and 0.139 centimeters, respectively. Only three plants increased in their leaf length; plants 1 (grown in wood), 6 (grown in stones), and 7 (control group). Both plants 1 and 6 increases in height by 0.1 centimeters, while plant 7 increased by 0.2
The first factor that I will be testing will be temperature. Different samples will be taken and grown under
Purpose: To see the effect of an acid introduced during seed germination, on the length of the plant roots. Also shows how salt can affect the seed germination. Acid can be introduced to seeds during germination if there is acid rain. Knowing the results of acid rain on seed germination will help us understand how to grow pants better, and how to have more successful germination. Salt can be introduced into a seed during germination because salt is put on roads and the salt builds up and can contaminate the soil. Knowing the effects of salt on seed germination will allow us to know for sure if the salt is affecting the plants growth or not.
The current study lacks analysis to the changes in root wall thickness. As we found the follow up period for one year was too short to produce a measurable change in the root walls thickness. A longer follow up period was needed to detect such changes. We
In this experiment it was found that the higher the salt concentration in a solution, the lower the mass of the Beetroot. As water travels out of the extracellular environment by Osmosis, the Cell began to dehydrate and signs of cell Lysis occurred. Though Colorimeter reading results were recorded, they did not align with the mass of the Beetroot data and were classified inaccurate. Reviewing the results there was no consistent trend in the data (eg. as the salt content increased by 1% the mass loss decreased by 0.5g). It could only be stated that as the salt concentration was increased the mass of the Beetroot decreased. This experiment is related to the Salinity issues currently occurring in Australia. It has been established that the rising levels of salt in soil affects a number of things like; Agricultural production, Water quality, Ecological health of streams, Terrestrial biodiversity, Soil erosion, Flood risk, Infrastructure and fixtures, irrigation. https://www.qld.gov.au/environment/land/soil/salinity/impacts/
Heterogeneity in phenotypic characters is a widely observed phenomenon by breeder’s in all plant species were bud grafting technique is adopted as the popular propagation technique. Though these variations are attributed to several internal and external factors, genetic heterogeneity of the root stocks is considered as one of the major contributor. It is presumed that a series of responses called stock scion interactions are triggered as a result of the grafting procedure where a genetically diverse stock and scion are joined together to form a single plant. The impact of these interactions on several aspects of plant growth and development is an area under extensive investigation for the past many years (Rogers and Beakbane 1957;