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Genetically Modified Organisms ( Gmos )

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This lab was performed to explore and discover GMOs in food that were given to test. Our group used oats, a GMO positive food, and a test food. Our goal was to find out whether the test food was GMO positive or negative and to record data accordingly. I hypothesized that the test food will be GMO positive. To see if my hypothesis had been correct we performed the process of PCR (Polymerase Chain Reaction), to find the presence of GMO positive or negative DNA in the foods. We first extracted the DNA from the foods, and then added primers to the DNA as well. I then inserted the DNA onto a gel using a micropipette. Using gel electrophoresis and a UV transilluminator we were able to view the staggering results. Genetically Modified Organisms …show more content…

But GMOs also have many adverse effects on health and the ecology of plants applied on. Genetically modified organisms disturb the ecological balance of their habitat through cross pollination. Cross-pollination is where two plants of different genetic backbone reproduce. Cross-pollination can occur over long distances as well. This allows new genes to be transferred to organic crops miles away. This makes it difficult to distinguish which crop field is organic, and which is not, posing a problem to the proper labeling of non-GMO food products. GMOs also carry unpredictable health effects for humans consuming these organisms. Our materials for the extraction of DNA included: the screwcap tubes, capless tubes, pipet tips, disposable transfer pipets, a foam microtube holder, a DI water bottle, a sharpie to label, a hot water bath, Non-GMO food, Test food, and a centrifuge. Our materials for the setup of the PCR reactions included: PCR tubes, screwcap tubes, pipet tips, an aerosol barrier, a foam microtube holder, a PCR tube holder, a 2-20 microliter micropipette, an ice bath, a sharpie to label, a plastic bag, a styrofoam cup with ice water, and a black tray with ice water. Finally, the materials for the electrophoresis of the PCR products included: Agarose gel, PCR sample tubes from previous labs, PCR tube holders, running buffers (300-350 ml), loading dye, a PCR molecular weight

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