Microbiology: An Introduction
11th Edition
ISBN: 9780321733603
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: Benjamin Cummings
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Chapter 20, Problem 6A
The following results were obtained from a broth dilution test for microbial susceptibility.
| Antibiotic Concentration | Growth | Growth in Subculture |
| 200 µg/ml | - | - |
| 100 µg/ml | - | + |
| 50 µg/ml | + | + |
| 25 µg/ml | + | + |
a. The MIC of this antibiotic is _______.
b. The MBC of this antibiotic is _______.
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Which one of the following methods of antimicrobial susceptibility testing combines some
of the properties of two other methods and involves adding a plastic strip to the agar?
Select one:
a. Agar macrodilution method
b. Gradient diffusion method
c. Broth microdilution method
d. Disk diffusion method
What results on a microdilution antimicrobial susceptibility test would indicate an
antibiotic would be an effective treatment for an infection?
O a. Growth next to the antibiotic
O b. High optical density in the well with inoculum and antibiotic
O c. Low optical density in the well with inoculum and antibiotic
O d. No growth next to the antibiotic disk
Clear my choice
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3-
Visible Growth in Tubes
Growth in Petri Dishes
0 µg/ml 2 µg/ml
0 1
3
4
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2
5 6 7
number 1, and
After addition of
The figure given above summarizes an experiment carried out to test the efficacy of
an antimicrobial substance. In the tube number 0, no antimicrobial substance was added
into bacterial culture. 2 µg/ml of antimicrobial substance was added into tube
the amount of antimicrobial was doubled for each consecutive tube.
antimicrobial into tubes, tubes were incubated for overnight and after incubation tubes were
observed for growth. The growth status of bacteria in each tube was shown as (+) and (-). In
addition to observation of visible growth, 1 ml of sample from each tube was collected and
plated into petri dishes and incubated for additional overnight. Similarly, the growth status in
petri dishes was as (+) and (-).
According to given information;
a. Determine the minimum inhibitory concentration (MIC).
b. Determine the minimum bactericidal concentration (MBC).
c.…
Chapter 20 Solutions
Microbiology: An Introduction
Ch. 20 - DRAW IT Show where the following antibiotics work:...Ch. 20 - List and explain five criteria used to identify an...Ch. 20 - What similar problems are encountered with...Ch. 20 - Define drug resistance. How is it produced? What...Ch. 20 - List the advantages of using two chemotherapeutic...Ch. 20 - Why does a cell die from the following...Ch. 20 - How does each of the following inhibit...Ch. 20 - The OH is missing from carbon 39 in ddI. How does...Ch. 20 - Compare the method of action of the following...Ch. 20 - NAME IT This microorganism is not susceptible to...
Ch. 20 - Which of the following pairs is mismatched? a....Ch. 20 - Prob. 2MCQCh. 20 - Prob. 3MCQCh. 20 - An antimicrobial agent should meet all of the...Ch. 20 - The most selective antimicrobial activity would be...Ch. 20 - Prob. 6MCQCh. 20 - Which of the following will not affect eukaryotic...Ch. 20 - Cell membrane damage causes death because a. the...Ch. 20 - A drug that intercalates into DNA has the...Ch. 20 - Chloramphenicol binds to the 50S portion of a...Ch. 20 - Prob. 1ACh. 20 - Why is idoxuridine effective if host cells also...Ch. 20 - Some bacteria become resistant to tetracycline...Ch. 20 - The following data were obtained from a...Ch. 20 - Prob. 5ACh. 20 - The following results were obtained from a broth...Ch. 20 - Vancomycin-resistant Enterococcus faecalis was...Ch. 20 - Prob. 2CAECh. 20 - Prob. 3CAE
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