Ribosomal RNA

Wellcome Trust Sir Henry Dale fellowship pre-application: Chih-Jen Lin Title: Acquisition of competence in the oocytes: epigenetic regulation of ribosomal RNA transcription. Background: The Oocyte, a highly differentiated cell type, has the remarkable capacity to reprogram cells (from sperm to even somatic cells) to that of totipotent embryonic cells. Understanding how oocyte-derived factors contribute to developmental competence will not only impact the fields of stem cell biology/cellular reprogramming

invasiveness. They also examined how a human diet can impact on invasion and microbial niche. In order to examine this, mice that were coprophagic, meaning they consume fecal matter, were housed together. Using Unweighted Uni-Frac analysis of 16S ribosomal RNA (rRNA) they found that the transplant recipient mice acquired the microbial species (microbiota) of the human donor efficiently. Furthermore, comparing enzyme commission numbers (ECs) of the genes using a shotgun sequencing-based approach, it was

ALPRAZOLAM Alprazolam is used as an anxiolytic drug and it has nonspecific depressant effects on the central nervous system. It’s used orally. It’s absorbed from gastrointestinal tact greater than 80%. Peak plasma levels usually occur within 2 hours. Alprazolam has a high protein bound (80%). Serum albumin accounts for the majority of the binding. It has a small volume of distribution (less than 1.5L/kg). Alprazolam and its metabolites are excreted primarily in the urine. The mean plasma elimination

The word Metrosideros is derived from two Greek words –“metra” which means “core or heart”, and “sideron” which means “iron”. Thus, Metrosideros species are referred to as iron-hearted trees referring to the durability and hardness of their wood (Dawson & Stemmermann, 1990). The genus Metrosideros belongs to the family Myrtaceae which consists of around 135 genera and more than 3000 species. A substantial number of species of this family are found in Australia and South America while fewer species

chicken host. With the host-specificity character, the Avibacterium genus was established within Pasteurellaceae family in 2005, including Avibacterium gallinarum, Avibacterium paragallinarum, Avibacterium avium and Avibacterium volantium. 16S ribosomal RNA analysis revealed that at least 96.8% sequence similarity was identified within this genus. This genus was also separable from all taxa

NIH Public Access Author Manuscript Nat Rev Microbiol. Author manuscript; available in PMC 2013 January 03. Published in final edited form as: Nat Rev Microbiol. 2011 April ; 9(4): 244–253. doi:10.1038/nrmicro2537. The skin microbiome $watermark-text Elizabeth A. Grice and Julia A. Segre Genetics and Molecular Biology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, 20892–4442, USA. Abstract $watermark-text The skin is the

Amplification of 16S Ribosomal DNA Sequences using Polymerase Chain Reaction Edwina Abou Haidar, Houssam Al Koussa, Mary AbedAlAhad. Department of Biology, Lebanese American University, Byblos, Lebanon Abstract The 16s rRNA gene sequencing is a widely common amplicon sequencing method used to identify and compare bacteria in a given sample. This method is well established and allows to study phylogeny and taxonomy of complex microbiomes. In this study, an unknown sample of extracted microbial

Shivani Patel BIO 398: Half-Credit Independent Research Annotated Bibliography 1. Seaton, S.C., Elliott, K.T., Cuff, L.E., Laniohan, N.S., Patel, P.R., and Neidle, E.L. 2012.   "Genome‐wide selection for increased copy number in Acinetobacter baylyi ADP1: locus and context‐dependent variation in gene amplification." Molecular microbiology. 83(3): 520-535. Gene duplication and amplification is a process by which variation can be created and selected for. By understanding gene duplication and amplification

richness in target environments can provide important validation for fine-tuning experimental and computational parameters used to characterize natural communities. Introduction For over two decades, amplification and sequencing of the small subunit ribosomal RNA (SSU rRNA or 16S rRNA) gene has been the primary approach to assess the abundance and taxonomic identity of microbes in the

RESEARCH ART I C L E Insights intothe dynamics of bacterial communities during chalcopyrite bioleaching Zhiguo He1,2, Fengling Gao1,2, Jiancun Zhao1,2, Yuehua Hu1,2 & Guanzhou Qiu1,2 1School of Minerals Processing and Bioengineering, Central South University, Changsha, Hunan, China; and 2Key Laboratory of Biometallurgy, Ministry of Education, Changsha, Hunan, China Correspondence: Zhiguo He, School of Minerals Processing and Bioengineering, Central South University, Changsha, Hunan 410083