Describe how Bacteria decode its genetic information to produce proteins?
Intro(10mins)
Bacteria belongs to a group of organism that lacks cell nucleus and membrane bound organells. This group of organisms are termed as prokaryotes. Prokaryotes follows the central dogma of molecular biology first proposed by Francis Crick in 1958 to synthesize proteins from mRNA through a process called translation and the mRNA is being synthesized from the DNA by another process called Transcription. Temperature, nutrient availibity are some key factors that start the process of synthesizing proteins in response to these key factors. Example. This paper will provide an explanation as to how bacteria decode the genetic information to produce
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Elongation is terminated by a stop codon. Stop codon do not code for any amino acid.
Protein folding
The amino acid sequences derived from decoding the mRNA determines a protein's final conformation, helper proteins aid the newly formed polypeptide with its folding to achieve a proper functional shape. These molecular chaperones are essential as the cytoplasm is often filled with new polypeptide chains and thus these accumulation of polypeptide chain might accumulate together and fold into a non-function shape. Example of well studied chaperones from E.Coli are DnaK, DnaJ, GroEL and GroES. And GrpE.
Protein splicing
Some microbial proteins are spliced after translation. In protein splicing, a part of the polypeptide is removed before folding to its final shape.
Conclusion
Decoding its genetic information refers to the process of transcription while producing proteins refer to the process of translation.
Body 1(30mins)
DNA transcription.
MRNA Translation
Body 2
Different between
Conclusion(10mins)
Introduction.
Bacteria belongs to a group of organism that lacks cell nucleus and other membrane bound organells. This group of organisms are called "Prokaryotes" and they follow the central dogma of molecular biology first proposed by Francis Crick in 1958 for protein synthesize. Protein synthesize is how instruction written by the bacteria DNA are being copied into a temporary form called mRNA and these mRNA are sent to
Transcription is the formation of an RNA strand from a DNA template within the nucleus of a cell. There are four nucleotides of DNA. These are adenine, cytosine, guanine and thymine. These nucleotides are transcribed to form messenger ribonucleic acid (mRNA) consisting of nucleotides made of adenine, cytosine, guanine and uracil. This transcription from DNA to mRNA happens by an RNA polymerase II. This newly created mRNA is read in the 5' to 3' direction in sets of 3. These sets are called codons. Each mRNA also has a cap and end. On the 5 prime side is a methylated guanine triphosphate and on the 3 prime is a poly A tail. Messenger RNA then moves to the cells cytoplasm and through the cells ribosomes for translation. Messenger RNA is matched to molecules of transfer RNA (tRNA) in the ribosomes to create amino acids. These amino acids subsequently form an amino acid chain. (Osuri, 2003) A visual representation of this can been viewed in figure 3.
3) As a ribosome moves along the mRNA, the genetic message is translated into a protein with a specific amino acid sequence.
After the DNA has been turned into mRNA a process called translation occurs and it turns the mRNA into tRNA.
Translation is a task that makes ribosomes synthesize proteins utilizing mRNA transcript made during transcription. In the begining of this task mRNA attaches it self to a ribosome so that it can be reveal a codon (three nucleotides).
RNA processing: In eukaryotic cells, introns, non-coding regions of RNA, are removed and a tail and a cap is added to RNA to help its movement.
Prior to the determination of the primary structure, the RNA strand undergoes splicing which alters the sequence. During splicing introns are removed and exons are randomly re-arranged in a random order. There is 2 problems with this model of attempting to estimate the protein a sequence will form from RNA. Firstly depending on the place of the body at which splicing is occurring, different introns will be removed and also secondly, we need to know which random order the exons will shuffle themselves into.
Our world has changed dramatically since the day Antoine van Leeuwenhoek discovered microorganisms in 1676 using a simple microscope. In early days, scientists first thought life arose from inanimate materials. This theory, known as abiogenesis or spontaneous generation, was disproved later on by scientists including Lazarro Spallanzani and Louis Pasteur. The experiments conducted by these scientists showed that living things could only arise from preexisting life, or biogenesis. All life begins with a living cell, composing of five required components. These components are DNA, RNA, cell membrane, ribosome, and cytoplasm. As more investigations on bacteria were conducted, scientists were able to acquire a deeper knowledge of the microbiology and pathology of animals, plants, and humans.
their normal shape to an abnormal shape, however, the chemical composition of the protein remains
Proteins are primarily considered to have one primary function to serve its role in an organism, however studies have observed to have multiple functioning proteins known as moonlighting proteins (Khan et al. 2014). Moonlighting proteins along with primary functions, have secondary functions that are not related to the primary function and does not correlate to the primary or other functions (Khan et al. 2014). The multifunctional proteins play essential roles in carrying out biochemical functions which aids in the cell growth but are not caused by gene fusion and multiple RNA splice variants (Amblee et al. 2015). The discovery of moonlighting proteins was first discovered by Piatigorsky and Wistow while observing crystallins (Khan et al. 2014). Crystallins, are structural proteins that are found in the eye lens that exhibit enzymatic activity to make the lens itself (Khan et al. 2014). Crystallin has a primary function to help form the lens of the eye by acting as a structural protein (Amblee et al. 2015). Besides enzymatic activity, crystallin was observed in other mammals to have secondary functions such as metabolic functions which are helpful in prokaryotic (Khan et al 2014). Most moonlighting proteins are characterized as cytosolic enzymes and chaperons, or in other words helping proteins (Amblee et al 2015). The multifunctional proteins or moonlighting proteins can also be identified as receptors, channel proteins and ribosomal proteins (Khan et al. 2014). Due to the
Proteins are polymeric chains that are built from monomers called amino acids. All structural and functional properties of proteins derive from the chemical properties of the polypeptide chain. There are four levels of protein structural organization: primary, secondary, tertiary, and quaternary. Primary structure is defined as the linear sequence of amino acids in a polypeptide chain. The secondary structure refers to certain regular geometric figures of the chain. Tertiary structure results from long-range contacts within the chain. The quaternary structure is the organization of protein subunits, or two or more independent polypeptide chains.
Protein synthesis is a two-part process that involves a second type of nucleic acid along with DNA. This second type of nucleic acid is RNA, ribonucleic acid.
Polypeptides’ creation is a long process that involves your DNA. A polypeptide is basically a protein that is bonded between amino acids. DNA triplets also involve in the creation of polypeptides. Inside the DNA the mRNA enters inside and copies the code. Since the DNA can’t leave the nucleus the mRNA is the messenger for the DNA. Which is called transcription since the mRNA copies the sequences and order of the bases. Then the DNA strands split up into two strands and an enzyme will encase it to be copied or let it be paired up with other bases. As the enzyme is replacing itself with nitrogen bases the second strand is being transferred out of the nucleus. The strands that are being transferred out of the nucleus can be paired up with tRNA
Bettelheim, Brown, Campbell and Farrell assert that polypeptide chains do not extend in straight lines but rather they fold in various ways and give rise to a large number of three-dimensional structures (594). This folding or conformation of amino acids in the localized regions of the polypeptide chains defines the secondary structure of proteins. The main force responsible for the secondary structure is the non-covalent
Transcription is where DNA is transcribed into RNA which then can be pass to the ribosome’s to act as a template for protein synthesis. Before transcription can begin DNA must unwind and the two halves of the molecule much come apart so exposing the base sequence. This process begins when a region of a two DNA strands is unzipped by enzyme called RNA polymerase attaches to the DNA molecule at the imitation site.
The formation of a protein begins in the genes, which contain the basic building information for all parts of living organisms. There are four DNA nucleotides that make up genes: A, T, C, and G. A codon is any arrangement of three of these nucleotides. Each triplet of nucleotides codes for one amino acid. First transcription will begin in the nucleus where mRNA will transcribe the DNA template. During both transcription and translation, there are three steps. The first step in transcription is initiation where RNA polymerase separates a DNA strand and binds RNA nucleotides to the DNA. RNA nucleotides are the same as DNA ones except that U replaces the T. The second is just the elongation of the mRNA. The third step of transcription is termination. This occurs when RNA polymerase reads a codon region and the mRNA separates from the