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- Eukaryotic Genetic Sequence: 5'-TAC CAT GAT CCC TAT - 3' 1. What would be the newly synthesized DNA strand and explain how the strand will be replicated. Where in the cell would this occur? 2. What would be the synthesized mRNA strand, and how is it transcribed from the original DNA strand, and then converted from a pre-mRNA strand to a mature mRNA? Where in the cell does this occur? 3. What would be the anti-codons for the tRNA. What are the amino acids generated based on the RNA. How are these amino acids translated into protein and where in the cell does this happen?Mammals contain a diploid genome consisting of at least 109 bp. If this amount of DNA is present as chromatin fibers, where each group of 200 bp of DNA is combined with 9 histones into a nucleosome and each group of 6 nucleosomes is combined into a solenoid, achieving a final packing ratio of 50, determine (a) the total number of nucleosomes in all fibers, (b) the total number of histone molecules combined with DNA in the diploid genome, and (c) the combined length of all fibers.The nuclear DNA content of a single sperm cell in Drosophila melanogasteris approximately 0.18 pg. What would be the expected nuclear DNA content of a primary spermatocyte in Drosophila? What would be the expected nuclear DNA content of a somatic cell (non-sex cell) in the G1 phase? What would be the expected nuclear DNA content of a somatic cell at metaphase?
- assuming a spacing of approxiametly 200 base pairs per nucleosome, how many molecules of histone H4 are present in one somatic human cell?Human Chromosome 22 (48 × 106 nucleotide pairs in length) has about 700 protein-coding genes, which average 19,000 nucleotide pairs in length and contain an average of 5.4 exons, each of which averages 266 nucleotide pairs. What fraction of the average protein-coding gene is converted into mRNA? What fraction of the chromosome do these genes occupy?A previously undiscovered single-cell organism was found living at a great depth on the ocean floor. Its nucleus contained only a single linear chromosome with 7 x 106 nucleotide pairs of DNA coalesced with three types of histone-like proteins. Consider the following questions: Question: The analysis of individual nucleosomes reveals that each unit contained one copy of each protein and that the shortlinker DNA contained no protein bound to it. If the entirechromosome consists of nucleosomes (discounting any linkerDNA), how many are there, and how many total proteins areneeded to form them?
- Assuming that the histone octamer forms a cylinder 9 nm in diameter and 5 nm in height and that the human genome forms 32 million nucleosomes, what volume of the nucleus (6 μm in diameter) is occupied by histone octamers?You would like to add a nuclear localization sequence (NLS) of Lys-Lys-Lys-Arg-Lys to a protein that is usually found in the cytoplasm of a yeast cell. To accomplish this, you introduce the nucleotide sequence encoding the NLS into the gene that encodes the cytoplasmic protein of interest. a. What is the size of the nucleotide insert that will encode the NLS? Briefly explain. 5' 3' b. Below is a diagram of the gene encoding the cytoplasmic protein of interest in the yeast genome. If your goal is to put the NLS at the carboxyl (C) terminus of the protein, at which location (A-E) should the NLS be inserted? Briefly explain. A TATAA ATATT promoter +1 B ATG TAC D TAA ATT stop codon E 3' 5'The chromosome 9/22 translocation associated withCML (chronic myelogenous leukemia) is called thePhiladelphia chromosome after the city in which itscancer association was first discovered in 1960.People with CML do not inherit this translocation—it occurs in somatic cells. Why do you think that thisparticular translocation that fuses the bcr and ablgenes happens independently in the somatic cellsof many different people?
- Recall that the nuclear membrane disintegrates late in prophase of mitosis in most eukarvotic cells. Once the membrane is reformed in telophase in a daughter cell, several components of gene expression mignt therefore be "caught" out in the cytoplasm when they would otherwise onlv ever be found inside the nucleus. Consider where the following components of gene expression are made and where they runction. Which component is normally never found in the cytoplasm outside the nucleus? O A. Spliced intron • B. RNA polymerase O C. Histones • D. DNA polymeraseThe value of "n" in the field mouse is 20. How many chromosomes are in a somatic mouse cell at the end of S phase? 0. 80 20 40Assuming that 32 million histone octamers arerequired to package the human genome, how many his-tone molecules must be transported per second pernuclear pore complex in cells whose nuclei contain 3000nuclear pores and are dividing once per day?