Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 17, Problem 63TQ
Summary Introduction
To review:
The explanation for the resistance of the histone cores to the hydrolytic actions of the nucleases.
Introduction:
DNA or deoxyribonucleic acid comprises 2 polydeoxynucleotidestrands, which are wrapped around one another in the form of the right-handed double helix. The structure of the DNA is known to be distinctive and hence, it is also referred to as double helix.
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Chapter 17 Solutions
Biochemistry: The Molecular Basis of Life
Ch. 17 - Prob. 1QCh. 17 - Prob. 2QCh. 17 - Prob. 3QCh. 17 - Prob. 4QCh. 17 - Prob. 5QCh. 17 - Prob. 6QCh. 17 - Prob. 7QCh. 17 - Prob. 8QCh. 17 - Prob. 1RQCh. 17 - Prob. 2RQ
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- Expressed sequences of DNA are called ________________.arrow_forwardQUESTION 8 Consider the pathway for the synthesis of the amino acid arginine in Neurospora: ARG-E → citrulline ARG-F ARG-H ornithine argininosuccinate arginine Mutant strains of Neurospora may carry one or more mutations. Neurospora mutant strain b is grown on minimal media plus supplements as shown. Growth is shown by (+) and no growth is shown by (o). Supplements ornithine mutant nothing citrulline arginino- succinate arginine strain What can you conclude from these data? O Strain a has only one mutation and it is in ARG-E. O Strain b has only one mutation and it is in ARG-H. O Strain a has a mutation in ARG-F and strain b has a mutation in ARG-E. O Strain b has mutations in ARG-E, ARG-F, and ARG-H. + +arrow_forwardNoncoding DNA sequences are called __________________.arrow_forward
- Sequence changes in DNA are called ______________.arrow_forwardEnzymes isolated from bacteria that cut DNA at specificsequences are called ____________________.arrow_forwardAn enzyme called _____________________ catalyzes theformation of a covalent phosphodiester bond between the 3′OH end of one DNA strand and the 5′ phosphate end of anadjacent strand during DNA replication.arrow_forward
- When nitrogenous bases in single-stranded polynucleotides are incorporated into doublestranded molecules, base pair formation results in altered UV light absorption. This is called the _____________ effectarrow_forwardA _____________ is a multienzyme complex that synthesizes RNA primers in E. coli DNA replication.arrow_forwardThe purpose of mitosis in human cells is for the production of two diploid, _________________ cells.arrow_forward
- A base change that has no discernible effect is called a____________________ mutation.arrow_forwardQuestion 7: Peptide nucleic acids (PNAS) exhibit greater stability as heteroduplexes with DNA (ie PNA-DNA duplex) than does double-stranded DNA (i.e. DNA-DNA duplex). However, peptide nucleic acids lack charged groups, making them largely insoluble under near-physiological conditions in aqueous buffer. Provide (1) an explanation for the increased stability of PNA-DNA duplexes (hint: consider intermolecular forces). (2) Additionally, propose modification(s) of the PNA scaffold DNA that could increase solubility without drastically reducing duplex stability. PNA R1 HN но. Base Base o=P-O NH Base Base 8 o=P-O- NH 8. Base Base НО NHarrow_forwardQuestion 5. AP1 is a transcription factor and an important regulator of gene expression and cell proliferation. Dysregulation of AP1 function may lead to cancer. The fos gene codes for AP1. Below is an experiment in which the role of a micro‐RNA (miR‐7b) in the regulation of fos gene expression was studied. miR‐7b shows partial sequence complementarity to the 3′‐untranslated region of fos mRNA. A DNA fragment coding for miR‐7b RNA and another fragment coding for a micro‐RNA unrelated to fos mRNA were cloned into vectors, and the recombinant plasmids (designated si‐miR‐7b, samples 3 and 4, and si‐miR‐neg, samples 5 and 6, respectively, in Fig. 1) were introduced into mouse fibroblast cells. Non-transfected cells were used as controls (samples 1 and 2). Samples 2, 4, and 6, were treated with a chemical PMA that induces transcription of fos gene; samples 1, 3, and 5 were left untreated. Two hours after PMA treatment protein extracts were prepared from the cultures and were then…arrow_forward
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