Principles of Biology
2nd Edition
ISBN: 9781259875120
Author: Robert Brooker, Eric P. Widmaier Dr., Linda Graham Dr. Ph.D., Peter Stiling Dr. Ph.D.
Publisher: McGraw-Hill Education
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Chapter 4, Problem 2TY
Summary Introduction
Introduction:
The technique of microscopy helps to identify micro-organisms and visualize various physiological features of the organism under study. Different types of microscopes are used for various purposes that allow easy detection and exhaustive study of the structure and properties of microbes.
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The field of view is the maximum area visible through the lenses of a microscope, and it is represented by a diameter. To determine the diameter of your field of view, you can place a transparent metric ruler under the low power (10X) objective of a microscope. You would focus the microscope on the scale of the ruler and measure the diameter of the field of vision in millimeters. Refer to the image taken of this preparation below. What is the approximate field of view as seen by this 10X objective. Note: This microscope has a 10X eyepiece, giving a total magnification of 100X. The image shows the 1mm is for this question.
- Answer for this question was 3mm.
The field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?
Which part of the microscope is used to adjust the light?
(pick one)
i.
h.
a.
d.
c.
Chapter 4 Solutions
Principles of Biology
Ch. 4.1 - With regard to the origin of life, why are...Ch. 4.1 - Prob. 2CCCh. 4.1 - Prob. 3CCCh. 4.1 - Prob. 1BCCh. 4.1 - Prob. 4CCCh. 4.1 - Prob. 1TYKCh. 4.1 - Prob. 2TYKCh. 4.1 - Prob. 3TYKCh. 4.2 - Prob. 1CCCh. 4.2 - Prob. 1TYK
Ch. 4.2 - Prob. 2CCCh. 4.2 - Prob. 2TYKCh. 4.3 - Prob. 1BCCh. 4.3 - Prob. 1CCCh. 4.3 - Prob. 2BCCh. 4.3 - Prob. 1TYKCh. 4.3 - Prob. 2TYKCh. 4.3 - Prob. 3TYKCh. 4.4 - Prob. 1BCCh. 4.4 - Prob. 1CCCh. 4.4 - Prob. 1TYKCh. 4.4 - Prob. 2BCCh. 4.4 - Prob. 2TYKCh. 4.4 - Prob. 3TYKCh. 4.5 - What is the function of the nuclear lamina and the...Ch. 4.5 - Prob. 1BCCh. 4.5 - Prob. 2CCCh. 4.5 - Prob. 1TYKCh. 4.5 - Prob. 2TYKCh. 4.5 - Prob. 3TYKCh. 4.6 - What is the advantage of having a highly...Ch. 4.6 - Prob. 1BCCh. 4.6 - Prob. 2CCCh. 4.6 - Prob. 1TYKCh. 4.6 - Prob. 2TYKCh. 4.7 - Prob. 1TYKCh. 4.8 - Prob. 1CCCh. 4.8 - Prob. 2CCCh. 4.8 - Prob. 3CCCh. 4.8 - Prob. 4CCCh. 4.8 - Prob. 1TYKCh. 4.8 - Prob. 2TYKCh. 4.8 - Prob. 3TYKCh. 4.9 - Which of the following could be found in a...Ch. 4.9 - Prob. 2TYKCh. 4 - The cell theory states that all living things are...Ch. 4 - Prob. 2TYCh. 4 - Prob. 3TYCh. 4 - Prob. 4TYCh. 4 - Prob. 5TYCh. 4 - Prob. 6TYCh. 4 - Prob. 7TYCh. 4 - Prob. 8TYCh. 4 - Prob. 9TYCh. 4 - Prob. 10TYCh. 4 - Describe two specific ways that protein-protein...Ch. 4 - Explain how motor proteins and cytoskeletal...Ch. 4 - A principle of biology is that structure...Ch. 4 - Prob. 1CBQCh. 4 - Prob. 2CBQ
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- During observation under the microscope, the number of optical planes that are in focus simultaneously define: The Working Distance The Field of View The Depth of Fieldarrow_forwardShown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.arrow_forwardExplain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?arrow_forward
- ewing Microscopic Objects" section to identify one way you can manipulate your slide to increase contrast AND one way you can make adjustment to the microscope itself to increase contrast. Copy the table below into your lab notebooks. Then, fill in the blanks using the information provided in the "Using our Microscopes" section (previous page) and he table atarrow_forwardWhich of the following is true about the relationship between depth of field and magnification? There is no relationship. When magnification increases, depth of field decreases. When magnification increases, depth of field increases. Stereomicroscopes offer a lesser depth of field than compound microscopes. The depth of field of a microscope determines how much magnification it can achieve.arrow_forwardWhich of the following statements are true in the context of the microscope's field of view? I. It is the observable area within a circular space as defined by the lenses II. FOV is the smallest on the lowest power objective III. FOV is the maximum area visible through the microscope eyepiece IV. When you switch to a higher power, the field of zooms out towards the centerarrow_forward
- True or False: Ultrathin sections are used for light microscopy but are to thin to be stable under an electron mocroscope X-ray beam.arrow_forwardYou have placed a microscope slide in a microscope and want to view it at with the 10X objective lens. List the steps that are necessary to set up and focus the microscope properly using the 10X objective lens.arrow_forwarda) Describe the technique Of Atomic Force Microscopy under the following headings: (i) Basic principle of AFM and overview of operations (ii) Modes of A FMarrow_forward
- Differentiate between the limit of resolution of the typical light microscope and that of the unaided human eyes the relationship between the depth of field and magnification is that When magnification increases, the depth of field decreases. Name two procedures (steps) you should do to achieve the maximum resolution when using the oil immersion lens State the relationship between the working distance of an objective lens and its magnification power “The coarse focus knob can be used to adjust the focus when using any of the objective lenses”. Is this statement true or false? If it is false, please correct it. When using the oil immersion lens but you cannot focus, what should you should not do? And what you should do? What does this statement mean: “Your microscope is parfocal”? What is the significance of condenser lens? Multiple choice question: The most useful adjustment for increasing image contrast in low power magnification is _______ Closing down the diaphragm Closing one…arrow_forwardTrue or false : each objective of a phase contrast microscope contains a phase plate. phase contrast microscopy relies on the condenser to increase the contrast of an image. in brightfield microscopy, closing the condenser aperture will decrease the contrast of the speciment. stained cell speciments are best viewed by phase contrast microscopy.arrow_forwardIn microscopy, why are different stains used for different samples? Be specific.arrow_forward
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