Principles of Biology
Principles of Biology
2nd Edition
ISBN: 9781259875120
Author: Robert Brooker, Eric P. Widmaier Dr., Linda Graham Dr. Ph.D., Peter Stiling Dr. Ph.D.
Publisher: McGraw-Hill Education
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Chapter 4.2, Problem 1TYK
Summary Introduction

Introduction:

Microscopy aids in the direct visualization and detection of various types of micro-organisms and thus allows their easy characterization and complete study. The micro-organisms can be detected in initial diagnosis of an infectious disease.

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Which of the following microscopes typically requires the use of vital dyes (like methylene blue) to visualize large subcellular structures in a living cell (like the nucleolus or the mitochondrion)? the scanning/tunneling electron microscope (STEM), with good resolution up to about 100,000,000x the transmission electron microscope (TEM), with good resolution up to about 100,000x the scanning electron microscope (SEM), with good resolution up to about 1,000,000x the compound light microscope, with good resolution up to about 1,500x all of the above microscopes would be equally useful in visualizing the interior of organelles
Types of Dyes/Stains According to Charge: Staining procedure Charge of the chromophore/ colored ion Charge of the target cellular structure Applications/Target cell structurelsites Dye Examples Positive Staining Negative staining
Microscope You should always position the cells or tissues in the center of view before increasing magnification, because you know that the image will move right as you move the slide to the left. you know that the image will move up as you move the slide down you know that the center of field of view will be unchanged this is the best way to avoid breaking the slide with an objective

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