Principles of Biology
2nd Edition
ISBN: 9781259875120
Author: Robert Brooker, Eric P. Widmaier Dr., Linda Graham Dr. Ph.D., Peter Stiling Dr. Ph.D.
Publisher: McGraw-Hill Education
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Chapter 4.2, Problem 1TYK
Summary Introduction
Introduction:
Microscopy aids in the direct visualization and detection of various types of micro-organisms and thus allows their easy characterization and complete study. The micro-organisms can be detected in initial diagnosis of an infectious disease.
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Which of the following microscopes typically requires the use of vital dyes (like methylene blue) to visualize large subcellular structures in a living cell (like the nucleolus or the mitochondrion)?
the scanning/tunneling electron microscope (STEM), with good resolution up to about 100,000,000x
the transmission electron microscope (TEM), with good resolution up to about 100,000x
the scanning electron microscope (SEM), with good resolution up to about 1,000,000x
the compound light microscope, with good resolution up to about 1,500x
all of the above microscopes would be equally useful in visualizing the interior of organelles
Types of Dyes/Stains According to Charge:
Staining
procedure
Charge of the
chromophore/
colored ion
Charge of the
target cellular
structure
Applications/Target
cell structurelsites
Dye
Examples
Positive
Staining
Negative
staining
Microscope
You should always position the cells or tissues in the center of view before increasing magnification, because
you know that the image will move right as you move the slide to the left.
you know that the image will move up as you move the slide down
you know that the center of field of view will be unchanged
this is the best way to avoid breaking the slide with an objective
Chapter 4 Solutions
Principles of Biology
Ch. 4.1 - With regard to the origin of life, why are...Ch. 4.1 - Prob. 2CCCh. 4.1 - Prob. 3CCCh. 4.1 - Prob. 1BCCh. 4.1 - Prob. 4CCCh. 4.1 - Prob. 1TYKCh. 4.1 - Prob. 2TYKCh. 4.1 - Prob. 3TYKCh. 4.2 - Prob. 1CCCh. 4.2 - Prob. 1TYK
Ch. 4.2 - Prob. 2CCCh. 4.2 - Prob. 2TYKCh. 4.3 - Prob. 1BCCh. 4.3 - Prob. 1CCCh. 4.3 - Prob. 2BCCh. 4.3 - Prob. 1TYKCh. 4.3 - Prob. 2TYKCh. 4.3 - Prob. 3TYKCh. 4.4 - Prob. 1BCCh. 4.4 - Prob. 1CCCh. 4.4 - Prob. 1TYKCh. 4.4 - Prob. 2BCCh. 4.4 - Prob. 2TYKCh. 4.4 - Prob. 3TYKCh. 4.5 - What is the function of the nuclear lamina and the...Ch. 4.5 - Prob. 1BCCh. 4.5 - Prob. 2CCCh. 4.5 - Prob. 1TYKCh. 4.5 - Prob. 2TYKCh. 4.5 - Prob. 3TYKCh. 4.6 - What is the advantage of having a highly...Ch. 4.6 - Prob. 1BCCh. 4.6 - Prob. 2CCCh. 4.6 - Prob. 1TYKCh. 4.6 - Prob. 2TYKCh. 4.7 - Prob. 1TYKCh. 4.8 - Prob. 1CCCh. 4.8 - Prob. 2CCCh. 4.8 - Prob. 3CCCh. 4.8 - Prob. 4CCCh. 4.8 - Prob. 1TYKCh. 4.8 - Prob. 2TYKCh. 4.8 - Prob. 3TYKCh. 4.9 - Which of the following could be found in a...Ch. 4.9 - Prob. 2TYKCh. 4 - The cell theory states that all living things are...Ch. 4 - Prob. 2TYCh. 4 - Prob. 3TYCh. 4 - Prob. 4TYCh. 4 - Prob. 5TYCh. 4 - Prob. 6TYCh. 4 - Prob. 7TYCh. 4 - Prob. 8TYCh. 4 - Prob. 9TYCh. 4 - Prob. 10TYCh. 4 - Describe two specific ways that protein-protein...Ch. 4 - Explain how motor proteins and cytoskeletal...Ch. 4 - A principle of biology is that structure...Ch. 4 - Prob. 1CBQCh. 4 - Prob. 2CBQ
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- During observation under the microscope, the number of optical planes that are in focus simultaneously define: The Working Distance The Field of View The Depth of Fieldarrow_forwardA student has a compound microscope equipped with 10X ocular lenses and 4X, 10X, 40X and 100X objective lenses. The student has a slide with cells that are -100 microns (1 micron = 103 mm) in diameter, and wants to view a magnified image of a %3D cell so that the cell appears to be 10 mm in diameter. Which objective lens should the student use? O 10X O 40X O 100X O 4X Question 7 2 ptsarrow_forwardFor each type of microscopy, know how each is used and the type of image produced. kEEP IT SHORT AND SWEET Light microscope – Transmission electron microscope - Scanning electron microscope –arrow_forward
- There are many different types of microscopes that can be used in science. Research the function of each type of microscope below Stereomicroscopes Bright field microscope Dark field microscope Fluorescence microscope Phase contrast microscope Nomarski microscope Scanning electron microscope Transmission electron microscopearrow_forwardWhat description best describes the compound light microscope? Uses a beam of electrons is transmitted through a specimen to form an image. Has more than one lens and its own light source. In this type of microscope, there are ocular lenses in the binocular eyepieces and objective lenses in a rotating nosepiece closer to the specimen. An optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. Designed for low magnification observation of a sample, typically using light reflected from the surface of an object rather than transmitted through it.arrow_forwardA student has a compound microscope equipped with 10X ocular lenses and 4X, 10X, 40X and 100X objective lenses. The student has a slide with cells that are -100 microns (1 micron = 10 mm) in diameter, and wants to viewa magnified image of a cell so that the cell appears to be 10 mm in diameter. Which objective lens should the student use? 10X O 40X O 100X 0 4Xarrow_forward
- These cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV. Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mmarrow_forwardWhy is cell imaging a preferred methos for studying cells?arrow_forwardWhich of the following statements regarding electron microscopy is FALSE? Question options: live organisms cannot be viewed with an electron microscope all of these statements are TRUE scanning electron microscopes focus electrons across the surface of a sample electrons pass through a sample viewed using transmission electron microscopy electron microscopes use magnetic fields in place of glass lenses electron microsopes have a better resolving power than light microscopesarrow_forward
- You can see your cells using the scanning and the 10x objective lenses. You cannot focus on anything when you add oil to your slide and move to the oil immersion objective. Your instructor comes over and wipes off your oil, turns over the slide, adds new oil, and moves the oil immersion objective into place. You can now focus on your cells. Knowing how immersion works, why couldn't you see the cells when the slide was upside down?arrow_forwardDescribe how images are formed under the microscopearrow_forwardHelp witharrow_forward
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